18th-20th April 2007University of Warwick, Coventry
Genetic Characterisation of Post Harvest Spoilage in Lettuce.
Atkinson, LD1., Buchanan-Wollaston, V1., Hand, P1., Schut, J2. and Pink, DAC1.
1Warwick HRI, University of Warwick, Wellesbourne, Warwick, Warwickshire,
2Rijk Zwaan, Burgemeester Crezeelann 40, Postbus 40, 2678 ZG De Lier Holland.
A major food industry concern is the post harvest discolouration of produce, as visual alteration incites unfavourable consumer responses. The shelf life of cut lettuce salad packs is regularly restricted by enzymatic browning and pinking via oxidation of polyphenols by polyphenol oxidase and accumulation of anthocyanins respectively. This project aims to provide breeding companies resources to improve cut lettuce shelf life through exploiting natural allelic variation for reduced browning and pinking.
A new linkage map based on selected F7 Saladin x Iceberg recombinant inbred lines (RILs) using fluorescent AFLPs and ESTs will be produced. Post harvest discolouration will be induced using a previously developed growing protocol and the F7s will be phenotypically assessed. QTL analysis for these traits will be carried out to identify potentially valuable markers. Associated metabolites will also be analysed in an attempt to identify potential biochemical markers for post harvest discolouration.
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