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Chloroplast immunity – a novel and key process attacked by pathogens

Primary Supervisor: Professor Murray Grant, School of Life Sciences

Secondary supervisor: Dr Alex Jones, SLS

PhD project title: Chloroplast immunity – a novel and key process attacked by pathogens.

University of Registration: University of Warwick

Project outline:

Biotic stress contributes disproportionately to crop losses, generally accounting for ~25% of yield. Thus, developing novel approaches to restricting pathogen infections of crops and consequently improving yields must be a key futurey objective.

This project is in the emerging field of chloroplast immunity. We recently discovered that the chloroplast is a key battleground in plant defence responses and is targeted by pathogens (de Torres et al. 2015).

The plant uses the chloroplast positively in defense, generating a burst of reactive oxygen species (ROS) on initial pathogen recognition. Successful pathogens deliver proteins (effectors) to suppress this ROS. More recent work has shown that the chloroplast it central to effective “gene-for-gene” (classic) resistance and even for generating long distance signals to activate systemic immunity. This project builds on these findings and the successful applicant will be at the forefront of research into understanding chloroplast immunity.

This multidisciplinary project will use proteomics, cell biology and genetic approaches to address the mechanistic basis of chloroplast mediated immunity. We will use new genetically encoded reporter lines to visualise the dynamics of ROS production and its suppression in real time during the infection process. We will use novel reporter lines to see how the chloroplast communicates with other organelles in the cell (mitochondria, ER, nucleus) during the infection process. And we will use both targeted and untargeted proteomic approaches to understand how chloroplast proteins are manipulated in this process, utilising the state-of-the-art proteomics facility in SLS.

The project will suit an enthusiastic and motivated student excited about making novel discoveries.


  1. de Torres-Zabala, et al. (2015) Photosynthesis is central to plant defence and pathogen effectors target the chloroplast Nature Plants doi:10.1038/nplants.2015.74
  2. Serrano et al. (2016) Chloroplasts at work during plant innate immunity. Journal of Experimental Botany, doi:10.1093/jxb/erw088

BBSRC Strategic Research Priority: Sustainable Agriculture and Food: Plant and Crop Science

    Techniques that will be undertaken during the project:

    • Microbiology – culturing bacterial and fungi, generating new bacterial lines with modified effectors, fundamental plant pathology techniques.
    • Whole plant imaging and data quantification using chlorophyll fluorescence, a key workhorse in monitoring chloroplast integrity.
    • Confocal imaging of traditional fluorescent and genetically encoded redox reporters.
    • Targeted and untargeted proteomics.
    • Basic plant genetics, developing mutant lines and selecting homozygous transgenic lines.

    Typical pattern of working hours:

    • 37.5 hrs per week, majority on Campus but flexibility available for data analysis.

    Contact: Professor Murray Grant, University of Warwick