Biological and Pharmaceutical Chemistry
Drug Design and synthesis
Design and synthesis of novel therapeutic agents for the prevention of diabetic complications and chemotheraphy of cancer. Modification of therapeutic agents to prolong therapeutic lifetime and enhance bioavailability.
Purification (enzymes, cytoskeletal protiens, receptors, immunoglobulins) and structural characterization.
Radiosynthesis and stable isotope-substituted synthesis. Use of 1H and 13C nuclear magnetic resonance, MALDI mass spectrometry, HPLC (preparative and analytical), absorption and fluorescence spectrophotometry.
Human and murine cell lines; isolation and culture of human blood cells (erythrocytes, neutrophils, monocytes, macrophages & lymphocytes) and rat hepatocytes.
AGEs and AGE REceptors
Studies of AGEs of proteins, nucleotides and phospholipids formed by glycation in vitro and in vivo. Study of AGE receptor epitope recognition, adaptor proteins and functional responses.
Cell dysfunction in hyperglycaemia
Assay of glycolytic intermediates and enzyme activities - particularly glyoxalases, transketolase and PKCβ, assay of α-oxoaldehydes and AGEs, expression of glyoxalases and transketolase.
Study by microscopy, flow cytometry and DNA electrophoresis. Caspase activities and inhibition, BID cleavage and JNK activation. Protein tyrosine kinase and phosphatase activity. Glutathione efflux. Death receptors and adaptor proteins.
Immunoassay, immunoprecipitation and immunoblotting, cell viability, cell growth, cell cycle, DNA, RNA and protein synthesis, glycolysis, immunofluorescence, Nothern blotting, DNA electrophoresis. Scintillation counting, electrophoresis and light microscopy.
Drug receptor binding. Mechanism of cation and efficacy studies. Chemical kinetics and pharmacokinetics.
Chemical modification for peptide drug delivery in vivo.
Studies of drug metabolism, pharmacokinetics and bioavailability.
Mechanisms of Disease (by collaboration)
Assessment of role in diabetic complications, Alzheimer's disease, uremia, alcoholism and ageing.
Chemoprevention and chemotherapy of cancer
Importance of dietary and pharaceutical isothiocyanates in primary and metastasis prevention. Glyoxalase I as a marker of tumourogenesis and a target for anticancer drug design.
Research Facilities, Instrumentation etc.
- Biochemistry/pharmacology/clinical chemistry laboratory
- Analytical MS laboratory with LC-MS/MS
- Cell culture suite
270 MHz NMR spectrometer, MALDI-MS, HPLC, UV/vis spectrophotometers, fluorimeter, microplate reader and washer.