Multidisciplinary research demands a broad range of technical expertise. My research team has the following technical expertise:
Quantitation of markers of proteins damage by glycation, oxidation and nitration by LC-MS/MS.
Metabolic mapping by LC-MS/MS.
Peptide mapping of protein damage by glycation, oxidation and nitration.
Proteomics of protein damage by glycation, oxidation and nitration.
Molecular modeling, kinetic and system modeling and bioinformatics
Structural modeling of proteins damaged by glycation, oxidation or nitration.
Kinetic, metabolic and pharmacokinetic modeling of protein damage and therapeutic intervention.
Use of protein informatics to predict functional sites in proteins susceptible to damage.
- Culture of human and murine cell lines. Isolation and culture of human blood cells (erythrocytes, neutrophils, monocytes, macrophages and lymphocytes). Isolation and culture of rat hepatocytes.
- Preparation and analysis of protein glycation adducts.
- Studies of the enzymatic defence against glycation.
Metabolic and cytological analysis (biochemical and clinical)
- Assay of enzyme activities (particularly glyoxalases, caspases, transketolase and other thiamine dependent enzymes), metabolic concentrations, immunoassay and immunoblotting, cell viability, cell growth, cell cycle, DNA, RNA and protein synthesis, glycolysis, immunoflourescence.
Signal transduction and apoptosis
Studies of tyrosine phosphorylation, protein tyrosine phosphatases, MAP kinases, transcription factors, caspase activities and inhibitors, and involvement of mitochondrial proteins.
Molecular biology techniques
cDNA propagation, vector customization, cell transfection for overexpression and siRNA gene silencing, PCR, real time RT-PCR, chromatin immunoprecipitation (ChIP), GFP-construct imaging, gene microarray and SNPs.
Synthetic biochemistry and physiological chemistry
Analytical standards for mass spectrometric assay of protein damage.
Analytical standards for mass spectrometric assay of physiological dicarbonyls.
Analytical standards for mass spectrometric assay of transcription factor activators.
Scintillation counting, liquid chromatography, gel electrophoresis, light electron microscopy, mass spectrometry, electron spin resonance, high resolution nuclear magnetic resonance, absorption & flourescence spectrophotometry, electrochemical techniques, Statistics, experimental design and power analysis.