WISB Research Career Development Fellow, 2017 to present
I investigate the practical application of bacteriophages (viruses of bacteria).
My research spans three main areas:
Applied microbiology and phage therapy
Phages have the potential to manipulate important microbial communities that are all around us, but these phages remain under-studied. In my environmental research I have studied ocean bacteria that produce oxygen, soil microbiomes that affect crop yield and freshwater processing. These are highly varied environments which harbor different bacterial communities, however bacterial functions are often conserved and the rules that govern these systems remain the same. Everywhere we find bacteria we also find their phages. Understanding the roles of these phages is key to unlocking their potential to shape these environments. I investigate how phages can be used to kill pathogenic bacteria and prevent or remove harmful and costly biofilms and biofouling.
My fellowship focuses on phage-host interaction in the gut microbiome and their impacts on human health and disease. I am interested in the impact of phages on general human health and the role they play in antibiotic resistance. This medical microbiology research focuses on gram negative bacteria, particularly Klebsiella and it's phages. Klebsiella is a World Health Organisation priority pathogen, with high levels of antimicrobial resistance, making it difficult to treat. My research has shown that Klebsiella produces trimethylamine (TMA) in the human gut, which contributes to health issues including cardiovascular disease and metabolic syndrome-associated. The substrates for TMA production come from the diet, particularly from meat and dairy products. The bacterial metabolic pathways for TMA production are understood for the pre-cursors choline, carnitine and glycine betaine and my research has revealed which bacteria contain these pathways. Phages are known to be in approximately equal proportions to bacteria in human feaces and have the potential to kill their bacterial hosts. There is a gap in current knowledge; gut phage and bacteria have been well catalogued, however there remains a lack of understanding of functional metabolism and phage-bacteria-host interactions.
Phages have the potential to be applied as an alternative or complement to antibiotics. I work with both aerobic and anaerobic culturing of bacteria both from the environment and clinical bacteria from hospitals. These bacterial cultures enable me to isolate phages that specifically target these bacteria.
Post-doctoral research fellow, The University of Warwick, 2012-2016. Anaerobic microbial degradation of quaternary amines to TMA. Contribution of TMA to methanogenesis in the environment and human health in the gut.
Post-doctoral research fellow, The University of Exeter, 2008-2011. Industrially funded through Shell. Bioengineering, using cyanobacteria for the production of biofuels.
Post-doctoral researcher, Bangor University, 2008. European BioMinE project. Bioengineering. Selective recovery metals from mine waste mediated by bacterial sulfidogenesis.
PhD, Plymouth Marine Laboratory/The University of Warwick, 2004-2008. Molecular studies on the diversity of the marine cyanobacteria Prochlorococcus and their co-occurring phages.
BSc, The University of Liverpool, Marine Biology, 1999-2002.
Memberships: SfAM and the Microbiology Society.
- Quareshy, Mussa, Shanmugam, Muralidharan, Townsend, Eleanor, Jameson, Eleanor, Bugg, Timothy D. H., Cameron, Alexander, Chen, Yin, 2021. Structural basis of carnitine monooxygenase CntA substrate specificity, inhibition and inter-subunit electron transfer. Journal of Biological Chemistry, 296
- Townsend, Eleanor, Kelly, Lucy, Muscatt, George, Box, Joshua D., Hargraves, Nicole, Lilley, Daniel, Jameson, Eleanor, 2021. The human gut phageome : origins and roles in the human gut microbiome. Frontiers in Cellular and Infection Microbiology, 11
- Townsend, Eleanor, Kelly, Lucy, Gannon, Lucy, Muscatt, George, Dunstan, Rhys, Michniewski, Slawomir, Sapkota, Hari, Kiljunen, Saija J., Kolsi, Anna, Skurnik, Mikael, Lithgow, Trevor, Millard, Andrew D., Jameson, Eleanor, 2021. Isolation and chacterisation of Klebsiella phages for phage therapy. PHAGE Therapy, Applications, and Research, 2 (1), pp. 26-42
- Gilbert, Rosalind A., Townsend, Eleanor M., Crew, Kathleen S., Hitch, Thomas C. A., Friedersdorff, Jessica C. A., Creevey, Christopher J., Pope, Phillip B., Ouwerkerk, Diane, Jameson, Eleanor, 2020. Rumen virus populations : technological advances enhancing current understanding. Frontiers in Microbiology, 11
- Townsend, Eleanor, Moat, John, Jameson, Eleanor, 2020. CAUTI's next top model : model dependent Klebsiella biofilm inhibition by bacteriophages and antimicrobials. Biofilm, 2
- Jones, Helen J., Kröber, Eileen, Stephenson, Jason, Mausz, Michaela A., Jameson, Eleanor, Millard, Andrew D., Purdy, Kevin J., Chen, Yin, 2019. A new family of uncultivated bacteria involved in methanogenesis from the ubiquitous osmolyte glycine betaine in coastal saltmarsh sediments. Microbiome, 7
- Michniewski, Slawomir, Redgwell, Tamsin, Grigonyte, Aurelija, Rihtman, Branko, Aguilo-Ferretjans, Maria, Christie-Oleza, Joseph, Jameson, Eleanor, Scanlan, David J., Millard, Andrew D., 2019. Riding the wave of genomics to investigate aquatic coliphage diversity and activity. Environmental Microbiology, 21 (6), pp. 2112-2128
- Jameson, Eleanor, Stephenson, Jason, Jones, Helen J., Millard, Andrew D., Kaster, K., Purdy, Kevin J., Airs, R., Murrell, J. C. (J. Colin), Chen, Yin, 2019. Deltaproteobacteria (Pelobacter) and Methanococcoides are responsible for choline-dependent methanogenesis in a coastal saltmarsh sediment. ISME Journal, 13 (2), pp. 277-289
- Jameson, Eleanor, Quareshy, Mussa, Chen, Yin, 2018. Methodological considerations for the identification of choline and carnitine-degrading bacteria in the gut. Methods, 149, pp. 42-48
- Jameson, Eleanor, Doxey, Andrew C., Airs, Ruth, Purdy, Kevin J., Murrell, J. C. (J. Colin), Chen, Yin, 2016. Metagenomic data-mining reveals contrasting microbial populations responsible for trimethylamine formation in human gut and marine ecosystems. Microbial Genomics, 2 (9)
- Jameson, Eleanor, Fu, Tiantian, Brown, Ian R., Paszkiewicz, Konrad, Purdy, Kevin J., Frank, Stefanie, Chen, Yin, 2015. Anaerobic choline metabolism in microcompartments promotes growth and swarming of Proteus mirabilis. Environmental Microbiology, 18, pp. 2886-2898
- Zhu, Yijun, Jameson, Eleanor, Cosatti, Marialuisa, Schäfer, Hendrik, Rajakumar, Kumar, Bugg, Tim, Chen, Yin, 2014. Carnitine metabolism to trimethylamine by an unusual Rieske-type oxygenase from human microbiota. Proceedings of the National Academy of Sciences of the United States of America, 111 (11), pp. 4268-4273
- Zhu, Yijun, Jameson, Eleanor, Parslow, Rosemary A., Lidbury, Ian, Fu, Tiantian, Dafforn, Tim, Schäfer, Hendrik, Chen, Yin, 2014. Identification and characterization of trimethylamine N-oxide (TMAO) demethylase and TMAO permease in Methylocella silvestris BL2. Environmental Microbiology, 16 (10), pp. 3318-3330
- Jameson, Eleanor, Mann, Nicholas H., Joint, Ian, Sambles, Christine, Mühling, Martin, 2011. The diversity of cyanomyovirus populations along a North?South Atlantic Ocean transect. The ISME Journal, 5 (11), pp. 1713-1721
- Jameson, Eleanor, Joint, Ian, Mann, Nicholas H., Muehling, Martin, 2010. Detailed analysis of the microdiversity of Prochlorococcus populations along a north-south Atlantic ocean transect. Environmental Microbiology, Vol.12 (No.1), pp. 156-171
- Jameson, E., Rowe, O. F., Hallberg, K. B., Johnson, D. B., 2010. Sulfidogenesis and selective precipitation of metals at low pH mediated by Acidithiobacillus spp. and acidophilic sulfate-reducing bacteria. Hydrometallurgy, 104 (3-4), pp. 488-493
- Johnson, D. Barrie, Jameson, E., Rowe, O. F., Wakeman, K., Hallberg, Kevin B., 2009. Sulfidogenesis at low pH by acidophilic bacteria and its potential for the selective recovery of transition metals from mine waters. Advanced Materials Research, 71-73, pp. 693-696
- Jameson, Eleanor, Joint, Ian, Mann, Nicholas H., Muehling, Martin, 2008. Application of a novel rpoC1-RFLP approach reveals that marine Prochlorococcus populations in the atlantic gyres are composed of greater microdiversity than previously described. Microbial Ecology, Vol.55 (No.1), pp. 141-151
- Jameson, Eleanor, Taubert, M., Coyotzi, S., Chen, Y., Eyice, O., Schafer, H., Murrell, J. C. (J. Colin), Neufelf, J. D., Dumont, M. G., 2017. DNA-, RNA-, and protein-based stable-isotope probing for high-throughput biomarker analysis of active microorganisms. In Streit, Wolfgang R.; Daniel, Rolf (eds.), Metagenomics : Methods and Protocols, Methods in Molecular Biology, Clifton, N.J, Humana Press ; Springer, pp. 57-74
|Title||Funder||Award start||Award end|
|Natural phages to inhibit bacterial-associated cardiovascular diseases (Eleanor Townsend)||Medical and Life Sciences Research Fund||01 Feb 2020||30 Jun 2021|
|Developing a clinically relevant phage cocktail against Klebsiella to prevent biofilm formation||BBSRC||01 Feb 2020||31 Oct 2020|