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Proteomics Research Technology Platform

Welcome to the Proteomics Research Technology Platform at the University of Warwick. We provide service and support to academic and commercial researchers seeking to identify and quantify proteins and their modifications. The analysis of protein mixtures from gel slices, co-immunoprecipitations or enrichments is routine and we can identify several thousand proteins in complex samples, for example from whole cell lysates or tissue extracts. Our favourite challenges include the analysis of protein phosphorylation and post-translational modification mapping. You will find support from an enthusiastic proteomics team to help with scientific discussion, experimental design, sample preparation and analysis of mass spectrometry data.

Covid-19 Statusgreen
  • currently operating at Phase 2 of return to work Risk Assessment (updated 29/06/2020)
  • Do NOT enter laboratory C1.15 without permission

Full details on the Proteomics RTP status page

Services

• Discussion and advice on experimental design and sample preparation for peptide-based mass spectrometry including enrichment strategies.

• Confirmation of protein identity, for example of recombinant proteins.

• Identification of protein interaction partners from co-immunoprecipitation experiments.

• Relative quantification of proteomes using label-free or stable isotope labelling (iTRAQ, TMT or SILAC).

• Analysis of protein modifications such as phosphorylation, acetylation or ubiquitination.

Laboratory Access

i. the SLS General Laboratory Induction

ii. the SLS Waste Management Moodle course

iii. the RTP Proteomics Laboratory Guidance Form .

Charges

We provide a fee-based service for Warwick researchers and external clients. Please contact us to discuss your requirements and to calculate costs

External customers must set up a contract with Warwick Scientific Services.

Instruments

• Orbitrap Fusion with UltiMate 3000 RSLCnano System (Thermo Scientific)

• Quantiva triple quadrupole with UltiMate 3000 RSLCnano System (Thermo Scientific)

• Xevo TQ with Acquity and NanoAcquity systems (Waters)

    Contact us

    Lab C115,
    School of Life Sciences,
    Gibbet Hill Road,
    University of Warwick,
    CV4 7AL

    Tel: 024 7615 0439
    proteomics@warwick.ac.uk


    People

    Alex Jones (Director)

    Andrew Bottrill (Manager)

    Cleidi Zampronio (Senior Technician)


    Learn More

    (PDF Document) What we do (Flyer)

    (PDF Document) What we do (Poster)

    (PDF Document) Proteomics Concepts Poster


    Training

    University of Warwick users can learn more about the service, and undertake training modules via the Proteomics Moodle


    "How to?" Video Guides

    Video Introduction to the lab

    Video protocol: Gel Digest

    Video protocol: In Solution Digest

    Video protocol: Stage Tip


    Reading Resources

    Essential background reading for those wanting a deeper understanding of Proteomics and Mass Spectrometry (library subscriptions required):

    Proteomics: Methods and Protocols

    Mass Spectrometry Data Analysis in Proteomics

    Introduction to Proteomics: Principles and Applications

    Mass Spectrometry: a Text Book