Welcome to the Proteomics Facility at the University of Warwick. We provide a full Proteomics Service to academic and commercial researchers seeking to identify and quantify proteins and their modifications. The analysis of protein mixtures from gel slices, co-immunoprecipitations or enrichments is routine and we can identify and quantify several thousand proteins in complex samples, for example from whole cell lysates or tissue extracts. You will find support from an enthusiastic proteomics team to help with scientific discussion, experimental design, sample preparation and analysis of mass spectrometry data.
• Discussion and advice on experimental design and sample preparation for peptide-based mass spectrometry including enrichment strategies.
• Confirmation of protein identity, for example of recombinant proteins.
• Identification of protein interaction partners from co-immunoprecipitation experiments.
• Relative quantification of proteomes using label-free or stable isotope labelling (iTRAQ, TMT or SILAC).
• Analysis of protein modifications such as phosphorylation, acetylation or ubiquitination.
Our proteomics core facility is equipped with state-of-the-art mass spectrometers and UPLC separation platforms for powerful LC-MS and LC-MS/MS.
• Left: timsTOF PROLink opens in a new window ion mobility Q-ToF mass spectrometer with nanoElute UPLC (Bruker)
• Centre: Orbitrap FusionLink opens in a new window with UltiMate 3000 RSLCnano System (Thermo Scientific)
• Right: QuantivaLink opens in a new window triple quadrupole with UltiMate 3000 RSLCnano System (Thermo Scientific)
We provide a fee-based service for Warwick researchers and external clients. Please contact us to discuss your requirements and to calculate costs.
External Clients should contact the laboratory manager to enquire about Project pricing. Contractual matters will be handled via Warwick Scientific ServicesLink opens in a new window.
Additional ways to access the Proteomics RTP:
- Seedcorn access is available to external PhD students through Warwick Analytical Science Centre.
- Talent-Led equipment sharing fund for technical staff.
- New users from Life Sciences must register for facility access - to be granted you will need to have completed:
- Visitors to Life Sciences (including Warwick Medical School staff/students) need to register as a Life Sciences Visitor in addition to the requirements above.
- Every job must have a completed sample submission form that contains full details for every sample.
School of Life Sciences,
Gibbet Hill Road,
University of Warwick,
Tel: 024 7615 0439
Alex Jones (Director)
Andrew Bottrill (Manager)
Cleidi Zampronio (Senior Technician)
University of Warwick users can learn more about the service, and undertake training modules via the Proteomics MoodleLink opens in a new window
"How to?" Video Guides
Essential background reading for those wanting a deeper understanding of Proteomics and Mass Spectrometry (library subscriptions required):