Proteomics Research Technology Platform
Welcome to the Proteomics Facility at the University of Warwick. We provide a full Proteomics Service to academic and commercial researchers seeking to identify and quantify proteins and their modifications. The analysis of protein mixtures from gel slices, co-immunoprecipitations or enrichments is routine and we can identify and quantify several thousand proteins in complex samples, for example from whole cell lysates or tissue extracts. You will find support from an enthusiastic proteomics team to help with scientific discussion, experimental design, sample preparation and analysis of mass spectrometry data.
Services
• Discussion and advice on experimental design and sample preparation for peptide-based mass spectrometry including enrichment strategies.
• Confirmation of protein identity, for example of recombinant proteins.
• Identification of protein interaction partners from co-immunoprecipitation experiments.
• Relative quantification of proteomes using label-free or stable isotope labelling (iTRAQ, TMT or SILAC).
• Analysis of protein modifications such as phosphorylation, acetylation or ubiquitination.
Instruments
Our proteomics core facility is equipped with state-of-the-art mass spectrometers and UPLC separation platforms for powerful LC-MS and LC-MS/MS.
• Left: timsTOF PROLink opens in a new window ion mobility Q-ToF mass spectrometer with nanoElute UPLC (Bruker)
• Centre: Orbitrap FusionLink opens in a new window with UltiMate 3000 RSLCnano System (Thermo Scientific)
• Right: QuantivaLink opens in a new window triple quadrupole with UltiMate 3000 RSLCnano System (Thermo Scientific)
Charges
We provide a fee-based service for Warwick researchers and external clients. Please contact us to discuss your requirements and to calculate costs.
Costs for University of Warwick staff and students
External Clients should contact the laboratory manager to enquire about Project pricing. Contractual matters will be handled via Warwick Scientific ServicesLink opens in a new window.
Additional ways to access the Proteomics RTP:
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- Seedcorn access is available to external PhD students through Warwick Analytical Science Centre.
- Talent-Led equipment sharing fund for technical staff.
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Laboratory Access
- New users from Life Sciences must register for facility access - to be granted you will need to have completed:
i. the SLS General Laboratory InductionLink opens in a new window
ii. the SLS Waste Management Moodle courseLink opens in a new window
iii. the RTP Proteomics Laboratory Guidance FormLink opens in a new window .
- Visitors to Life Sciences (including Warwick Medical School staff/students) need to register as a Life Sciences Visitor in addition to the requirements above.
- Every job must have a completed sample submission form that contains full details for every sample.
Contact us
Lab C115,
School of Life Sciences,
Gibbet Hill Road,
University of Warwick,
CV4 7AL
Tel: 024 7615 0439
proteomics@warwick.ac.uk
People
Alex Jones (Director)
Andrew Bottrill (Manager)
Cleidi Zampronio (Senior Technician)
Brochure
Link opens in a new window
Learn More
What we do (Flyer)Link opens in a new window
What we do (Poster)Link opens in a new window
Proteomics Concepts PosterLink opens in a new window
Training
University of Warwick users can learn more about the service, and undertake training modules via the Proteomics MoodleLink opens in a new window
"How to?" Video Guides
Video Introduction to the labLink opens in a new window
Video protocol: Gel DigestLink opens in a new window
Video protocol: In Solution DigestLink opens in a new window
Video protocol: FASP DigestLink opens in a new window
Video protocol: Stage TipLink opens in a new window
Reading Resources
Essential background reading for those wanting a deeper understanding of Proteomics and Mass Spectrometry (library subscriptions required):
Proteomics: Methods and ProtocolsLink opens in a new window
Mass Spectrometry Data Analysis in ProteomicsLink opens in a new window
Introduction to Proteomics: Principles and ApplicationsLink opens in a new window