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 Preliminary Experiment

 

December 2009

Preliminary experiment to determine whether 'wild-type' and resistance-breaking (Rb) N. ribisnigricollected in the UK display any developmental and behavioural differences when reared on susceptible and resistant lettuce varieties.

This was an initial experiment to determine development rates and intra-plant distribution of ‘wild-type’ and resistance-breaking aphids on resistant and susceptible lettuce plants. A sub-objective was to develop a protocol for studying aphid development and behaviour in subsequent experiments.

Developmental rates experiment

 

March 2010 - January 2011

Developmental rates experiment

To determine the development rates and intra-plant distribution of ‘wild-type’ and resistance-breaking aphids on resistant and susceptible lettuce plants at a range of different temperatures.

 Completed field trial layout 2

 

June 2010- October 2010

Aphid mid-summer crash preliminary field experiment

To determine whether entomopathogenic fungi and natural enemies play a role in the mid-summer aphid crash by having various netted plots and open plots with various fungicide and/or insecticide treatment regimes.

 

 

Picture of Chicory Infested with Nasonovia

 

August- November 2011

Alternative host plant experiment

To determine what alternative plant hosts resistance breaking and wild type N. ribisnigri can survive to adulthood at 20°C (18L 6D).


 

 

 field trial 2011

 

May 2011 - October 2011

Aphid mid-summer crash experiment 2011

To determine whether entomopathogenic fungi and natural enemies play a role in the mid-summer aphid crash. The method has been adapted from the preliminary field trial which took place spring Summer 2010.

 male and asexual winged Nasonovia

 

March 2011 - present

Inducing the production of sexual aphids (males and oviparae) to obtain overwintering eggs

Different light and temperature regimes have been used to simulate autumn conditions and induce the production of sexual males and females which can then be used to obtain overwintering eggs.

 

 

 overwintering weed experiment

 

November 2011- March 2012

Overwintering Alternative hosts

Four alternative hosts to lettuce were inoculated with N. ribisnigri and planted in sheltered and unsheltered plots to determine whether N. ribisnigri can overwinter.

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