Exploiting Next Generation Sequencing to invstigate the genetics of parsnip
root disease and develop a marker assisted breeding strategy.
To develop tools to faciliate breeding for quantitative resistance to parsnip root canker diseases caused by Itersonilia and Mycocentrospora spp.
Parsnip Breeding Lines
Parsnips (Pastinaca sativa) are a speciality crop within the UK, covering an area of around 4100ha and a value of £64M annually. Currently the major constraint to production is the losses associated with root canker diseases caused by fungal pathogens such as Itersonilia and Mycocentrospora spp.
This project aims to address this problem by facilitating breeding for quantitative resistance to these major diseases. Some resistance to parsnip canker exists but is difficult to select for using traditional phenotype screening methods. The main objectives are to further understand the epidemiology of the pathogens involved in parsnip cankers; to develop specific plant bioassays to identify resistant parsnip breeding lines and develop markers for mapping quantitative trait loci (QTL) conferring resistance.
Resistance screening assays have been developed using both parsnip seedlings and roots. In a root assay using 96 parsnip breeding lines and 10 parent lines, a large range of resistance responses to Itersonilia and Mycocentrospora was observed amongst different lines. A further SNP-genotyped parsnip mapping population will be screened to try and determine the QTLs conferring resistance.
The project will have a direct impact upon the industry by enabling improved sustainability of parsnip production in the UK.
Discard Parsnip roots from processing factory.
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