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Curriculum Vitae

Education and Research Experience

2014 - 2019: PhD in Biological Sciences (BBSRC funded with MIBTP at The University of Warwick, Coventry, UK)

Project: A novel linkage between protein translational fidelity control and bacterial cell wall biosynthesis

Mini Project 1: University of Birmingham, Edgbaston, UK

Project: Purification and Crystal Screening of MglABd-Bd2492, Bd2492 and RomR mutants

Supervisor:Dr Andrew Lovering

Mini Project 2 University of Warwick, Coventry, UK

Project: Kinetic investigations into substrate analogues of MurF as potential antimicrobials

Supervisors:Professor David Roper and Dr Adrian Lloyd

Professional Industrial Placement: Cawthron Institute, Nelson, NZ

Project: To create a Microsoft Excel programme using BASIC for automation of School Science Fair event within the Community and Education Team

2010 - 2014: BSc in Biomedical Science with Intercalated Year (The University of Warwick, Coventry, UK)

Overall grade: 76% (1st class honours classification)

2012: Intercalated Year Placement (Monoclonal Antibiody Group, GlaxoSmithKline, Stevenage)

Project: Generating a panel of monoclonal antibodies towards a proto-oncogenic transcription factor

2011: Summer Placement (School of Life Sciences, University of Warwick)

 Project: Corruption of peptidoglycan synthesis in Streptococcus pneumoniae

Skills and Techniques

Cellular biology: Aseptic technique and tissue culture of routine cell lines (HEK293, BL21), CAT2 tissue culture (Streptococcus pneumoniae, Staphylococcus aureus), recombinant protein expression, susceptibility testing (MIC)

Molecular Biology: Cloning, site directed mutagenesis, protein purification, DNA/RNA extraction, in vitro transcription, PCR, Next generation sequencing (NGS)

Protein Biochemistry: FPLC,Western-blot, ELISA, UV/Vis spectrophotometry, fluorescence anisotropy, protein crystallography, mass spectrometry, radioactivity assays, enzymology

Computational: MATLAB, R, Visual Basic for Applications (VBA), LaTeX, PyMOL