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Location MCBb L1.11
Owner Balasubramanian group
Trainer Anton Kamnev

Andor Revolution XD system is a spinning disk confocal fluorescent microscope. The microscope is based on Nikon Eclipse-Ti fully motorized inverted base and is equipped with Yokogawa CSU-X confocal unit and high sensitivity EMCCD camera. Full heated enclosure ensures control of the sample temperate and reduces thermal drifts. Light path of the system is fully motorized which allows rapid imaging of multiple fluorescent/bright field channels over long period of time. In addition, the system is equipped with CellAsic perfusion system.

Spinning disk confocal allows fast (up to 50 fps) capture of submicron optical sections of the sample. Reduction of out of focus light allows this microscope to resolve objects as small as hundreds of nm (300~500 nm in X/Y and ~600 nm in Z). Moreover, reduction of excitation area during imaging leads to minimal photodamage of the sample. Fast acquisition combined with high resolution and low photodamage makes spinning disk microscopes leading tool for investigation of processes on submicron level in both fixed and live samples.


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Base type Nikon Eclipse-Ti, inverted
Bright field BF
Objectives Oil: 100x, 60x, 40x
Air: 20x
Confocal fluorescence Type: spinning disk confocal
Channels: Dapi, GFP, Cy3/mCherry, Cy5
Laser lines: 405, 488, 561, 640 nm
Epifluorescence
(eye piece only)
Source: White LED (MonoLED)
Channels: Dapi, GFP, Cy3/mCherry
Detector EMCCD (Andor iXon Ultra)
Motorized stage Z: Piezo stage, 400 μm travel range
XY: Motorized stage
Temperature control Heating: Full enclosure
Default temp: RT
Stage inc: -
Additional features CellAsic perfusion system