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Location MCBb L1.11
Owner Balasubramanian group
Trainer Anton Kamnev

Andor Revolution system is an epifluorescent light microscope with DIC capabilities. The microscope is based on Nikon Eclipse-Ti fully motorized inverted base and is fine tuned for imaging of live yeast cells over long periods of time. Full heated enclosure ensures control of the sample temperate and reduces thermal drifts. Light path of the system is fully motorized which allows rapid imaging of multiple fluorescent/bright field channels over long period of time. In addition the system is equipped with LED excitation source, which minimizes photo damage and increases efficiency of fluorescent imaging.

Wide-field or epi-fluorescence microscope is the most common tool in bioimaging. Due to its low cost and relatively simple imaging process wide-field systems are the primary choice for routine imaging of mm~μm scale objects (embryos, cells, cell organelles). Moreover, resolution limit of the system could be improved by post-acquisition removal of out of focus blur (deconvolution). Resolution of properly deconvolved image could be comparable with confocal systems (300~500 nm in XY and ~600 nm in Z).


Base type Nikon Eclipse-Ti, inverted
Bright field BF, DIC
Objectives Oil: 100x, 40x
Air: 20x, 10x
Fluorescence Type: epifluorescence
Channels: Dapi, GFP, Cy3/mCherry, Cy5
Light source: X-Cite XLED-1
Detector sCMOS (Andor Zyla)
Motorized stage Z: Piezo stage, 400 μm travel range
XY: Motorized stage
Temperature control Heating: Full enclosure
Default temp: RT
Stage inc: -
Additional features CellAsic perfusion system