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Location	MCBb L1.22
Owner	Millar group
Trainer	Anton Kamnev

Nikon Eclipse TE2000-E system is an epifluorescent light microscope with DIC capabilities. The microscope is based on semi-motorized inverted base and is fine tuned for imaging of live yeast cells over long periods of time. Full heated enclosure ensures control of the sample temperate and reduces thermal drifts. Light path of the system is fully motorized which allows rapid imaging of multiple fluorescent channels over long period of time.

Wide-field or epi-fluorescence microscope is the most common tool in bioimaging. Due to its low cost and relatively simple imaging process wide-field systems are the primary choice for routine imaging of mm~μm scale objects (embryos, cells, cell organelles). Moreover, resolution limit of the system could be improved by post-acquisition removal of out of focus blur (deconvolution). Resolution of properly deconvolved image could be comparable with confocal systems (300~500 nm in XY and ~600 nm in Z).

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Base type	Nikon Eclipse TE2000-E, inverted
Bright field	BF, DIC
Objectives	Oil: 100x
	Air: 40x, 10x
Fluorescence	Type: epifluorescence
	Channels: Dapi, GFP, Cy3/mCherry, Cy5
	Light source: Xenon lamp
Detectors	CCD (Photometrics CoolSNAP HQ2)
	EMCCD (Rolera EMC2)
Motorized stage	Z: Piezo stage, 100 μm travel range
	XY: Motorized stage
Temperature control	Heating: Full enclosure
	Default temp: RT
	Stage inc: -
Additional features	-