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Location MCBb L1.10
Owner Royle group
Trainer Steve Royle

Olympus IX71 system is an epifluorescent light microscope. The microscope is based on inverted base and equipped with full heated enclosure, which ensures control of the sample temperate and reduces thermal drifts. In addition system is equipped with perfusion system and electrical stimulation module for patch clamp experiments.

Wide-field or epi-fluorescence microscope is the most common tool in bioimaging. Due to its low cost and relatively simple imaging process wide-field systems are the primary choice for routine imaging of mm~μm scale objects (embryos, cells, cell organelles). Moreover, resolution limit of the system could be improved by post-acquisition removal of out of focus blur (deconvolution). Resolution of properly deconvolved image could be comparable with confocal systems (300~500 nm in XY and ~600 nm in Z).


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Base type Olympus iX71, inverted
Bright field BF
Objectives Oil: 100x, 60x, 40x
Air: -
Fluorescence Type: epifluorescence
Channels: GFP, pHluorin, mCherry
Light source: Hg lamp (Olympus MT20)
Detector CCD (Hamamatsu Orca-ER)
Motorized stage Z: -
XY: -
Temperature control Heating: Full enclosure
Default temp: RT
Stage inc: -
Additional features Perfusion system
Electrical stimulation