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| Location | MCBb L1.18 |
|---|---|
| Owners | Cross, McAinsh & Straube groups |
| Trainer | Anne Straube |
OOlympus TIRF-2 FRAP system is based on Olympus IX81 inverted base and equipped with epi-fluorescence, multiline TIRF and FRAP modules. Light path of the system is fully motorized which allows rapid imaging of multiple TIRF/epi-fluorescent/BF channels over long periods of time.
Total Internal Reflection Fluorescence (TIRF) technique allows selective illumination of fluorescent tags localized very close to the coverslip surface (0~300 nm). Selective illumination of such a thin layer of sample leads to dramatic increase of contrast allowing imaging of very dim signals at high temporal resolution (e.g. single molecule 2D dynamics, microtubule dynamics assays, focal adhesion in cell migration etc.).
| Base type | Olympus IX81, inverted |
| Bright field | BF |
| Objectives | Oil: 100x |
| Air: - | |
| Fluorescence | Types: epi-fluorescence & TIRF |
| Channels: Dapi, GFP, Cy3/mCherry, Cy5 | |
| TIRF laser lines: 488, 561 & 640 nm | |
| Epi light source: Metal halide lamp (Olympus MT20) | |
| Detectors | EMCCD (Hamamatsu ImagEM-1K) |
| Motorized stage | Z: Objective motor |
| XY: manual control | |
| Temperature control | Heating: Full enclosure |
| Default temp: - | |
| Stage inc: - | |
| Additional features | FRAP module (405 nm) |
| Detector beamsplitter (dual acquisition mode) |