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Antiproliferative activity screening services

Mammalian cell culture techniques are routinely employed to study the biological properties of novel compounds towards cell lines in vitro. Typically, activities are determined by treatment of cells with different concentrations of the test sample, and cell survival (%) is determined using an appropriate biochemical assay.
We have a wealth of experience and strong track record of published research output in this area and would be happy to discuss your requirements during all stages of the experiment.
 

cogs Applications:

  • Assessment of antiproliferative activity towards various human cell lines (e.g. carcinomas, primary cell lines)
  • Quantify biological activity as part of on-going drug development or toxicity studies.
  • More sophisticated mechanistic experiments (for example; cell cycle check, induction of apoptosis)
  • Typical samples: organic compounds, metal complexes, macromolecules, nanoparticles...

cogs How does it work?

After selection of an appropriate and relevant cell line, cells are maintained in vitro. Cells are then exposed to a specific test concentration range for a defined time period. After this time, cell viability (survival) is determined using an assay that is both appropriate and compatible with the original sample.

Experiments are typically conducted with adherent cell lines in a 96-well plate format, however suspension cell lines can be used if required. Cell viability assays are carried out with assistance from robot liquid handling automation to enhance reproducibility. End-point assays typically determine cell survival using colorimetric techniques that either rely on cellular function (MTT/XTT) or by protein quantification (SRB), however this experimental design can be modified during planning stages of consultation.

cogs Sample handling requirements:

The sample must be of a known purity (typically > 98%) and soluble in either aqueous media (e.g. PBS, water, cell culture medium) or in the presence of a non-toxic concentration of DMSO/DMF (< 5% v/v). Typically, no more than 5 mg of sample is required. The sample must be compatible with aqueous solvents. We recommend that aqueous stability studies are carried out before sample submission. Please feel free to ask if you are unsure.

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Contact:
Dr Ian Hancox, 024 76 150 380
email i.hancox@warwick.ac.uk

Dr James Coverdale
email j.coverdale@warwick.ac.uk
Typical results format, and sample:
Status
Availability
green_tick.gif Warwick collect/analyse data
green_tick.gif Warwick collect data
 green_tick.gif Available to user with expertise/ contribution
 

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