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BEGIN:VEVENT
DTSTAMP:20260410T001954Z
DTSTART;VALUE=DATE-TIME:20250922T133000
DTEND;VALUE=DATE-TIME:20250922T150000
SUMMARY:Cellular Interfaces Seminar: My Travels with Salmonella and Mike 
 Sheetz: The Use of Low Frequency Ultrasound to treat Immune Senescence a
 nd other Age-related Maladies\, Professor Linda J. Kenney\, Mechanobiolo
 gics
TZID:Europe/London
UID:20250922-8ac672c4994b53fb01995256a6af2bc0@warwick.ac.uk
CREATED:20250916T114354Z
DESCRIPTION:Abstract: Accurately measuring fluctuating pH levels in bacte
 rial cells is vital for understanding their signaling pathways and patho
 genesis\, which is hindered by the lack of effective pH probes. We emplo
 yed mCherryTYG\, a genetically-encoded fluorescent probe\, coupled with 
 Fluorescence Lifetime Imaging Microscopy (FLIM) to map pH changes in bac
 teria and their associated bacterial lifestyles. We measured pH fluctuat
 ions in diverse environments\, including Salmonella-containing vacuoles\
 , in biofilms\, and in heterologous hosts the nematode C. elegans\, and 
 zebrafish Danio rerio1. Salmonella enterica serovar Typhi is the causati
 ve agent of typhoid fever\, a human-restricted systemic infection. We co
 mpared the H58 response (a rapidly expanding serovar) in vitro and durin
 g infection of THP-1 human macrophages with the well-characterized Salmo
 nella Typhimurium\, which causes gastroenteritis. In response to acid st
 ress\, the sulfur assimilation pathway was highly upregulated and unique
  to H58. In acidic pH\, H58 was more oxidized\; the sulfur assimilation 
 pathway mitigated the redox stress. We emphasize the importance of study
 ing S. Typhi strains directly to understand their unique behavior during
  pathogenesis2. The presence of senescent cells causes age-related patho
 logies\, since their removal by genetic or pharmacological means improve
 s outcomes in animal models. An alternative to depleting such cells is t
 o rejuvenate them to promote their return to a replicative state. Treatm
 ent of non-growing senescent cells with low-frequency ultrasound (LFU) r
 ejuvenated cells for growth. Notably\, 15 characteristics of senescent c
 ells were reversed by LFU. Mechanistically\, LFU causes Ca2+ entry and i
 ncreased actin dynamics\, increases autophagy and inhibits mTORC1 signal
 ing. LFU treatment increased mouse lifespan and healthspan3. Mice fed a 
 high fat\, high sucrose diet combined with streptozotocin developed insu
 lin resistance and diabetes. Transcriptomic analysis indicated that LFU 
 primarily reduced inflammatory and immune-related gene expression\, by p
 romoting a shift toward an anti-inflammatory (M2) macrophage profile4. E
 lderly populations are susceptible to infection due to immune senescence
 \; we explored the effect of LFU prior to infecting aged mice with Salmo
 nella. Colonization was significantly reduced when old mice were treated
  with LFU. Chemokine analysis revealed a significant increase in ICAM-1\
 , SDF-1\, and KC/CXCL1 in aged\, treated mice. Ultrasound treatment reju
 venated the immune system in old mice\, reducing their susceptibility to
  Salmonella infection5. Taken together\, these results indicate that mec
 hanically induced pressure waves alone can reverse senescence and aging 
 effects at the cellular and organismal level\, providing a non-pharmacol
 ogical way to treat the effects of aging. 1. Singh\, M. K.\; Fernandez\,
  M.\; Dilawari\, R.\; Zangoui\, P.\; Kenney\, L. J.\, FLIM of mCherryTYG
  Deciphers pH Dynamics and Lifestyles of Salmonella Typhimurium. ACS Sen
 s 2025\, 10 (6)\, 4232-4243. 2. Fernandez\, M.\; Yamanaka\, Y.\; Zangoui
 \, P.\; White\, M. A.\; Kenney\, L. J.\, The sulfur assimilation pathway
  mitigates redox stress from acidic pH in Salmonella Typhi H58. mBio 202
 5\, 16 (7)\, e0046725. 3. Kureel\, S. K.\; Maroto\, R.\; Aniqua\, M.\; P
 owell\, S.\; Singh\, E.\; Margadant\, F.\; Blair\, B.\; Rasmussen\, B. B
 .\; Sheetz\, M. P.\, Rejuvenation of Senescent Cells\, In Vitro and In V
 ivo\, by Low-Frequency Ultrasound. Aging Cell 2025\, e70008. 4. Marchant
 \, E. D.\; Singh\, E.\; Kureel\, S.\; Blair\, B.\; Kalenta\, H.\; Von Ru
 ff\, Z. D.\; Weldon\, K. S.\; Lai\, Z.\; Sheetz\, M. P.\; Rasmussen\, B.
  B.\, Low-Frequency Ultrasound Reverses Insulin Resistance and Diabetes 
 Induced Changes in the Muscle Transcriptome in Aged Mice. Am J Physiol E
 ndocrinol Metab 2025. 5. Zangoui\, P.\; Singh\, E.\; Sheetz\, M. P.\; Ke
 nney\, L. J.\, Low-frequency ultrasound treatment reduces susceptibility
  to Salmonella infection in aged mice. J Bacteriol 2025\, e0017625.
LOCATION:GLT1\, Medical School Building
URL:
ATTACH:
CATEGORIES:BiomedicalSciences,Cellular Interfaces Seminar,Directorate Sem
 inars
LAST-MODIFIED:20250916T114354Z
ORGANIZER;CN=Jas Bains:
END:VEVENT
END:VCALENDAR