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Techniques Used

In no particular order, methods used in my research:

  • Stable-isotope probing (SIP)
  • Fluorescent in situ hybridisation (FISH)
  • Quantitative-microautoradiography coupled to fluorescent in situ hybridisation (Q-MAR-FISH)
  • Denaturing gradient gel electrophoresis (DGGE)
  • Enzyme assays
  • Analytical chemistry
  • Inductively coupled plasma atomic emission spectroscopy (ICP-OES)
  • Continuous culture (substrate-limited chemostats)
  • Polyacrylamide gel electrophoresis of proteins (SDS-PAGE, Native PAGE, BN-PAGE, CN-PAGE and NuPAGE)
  • Gas chromatography (GC)
  • Thin-layer chromatography (TLC)
  • Paper chromatography (it might be old-fashioned but it works!)
  • Polymerase chain reaction (PCR)