Techniques Used
In no particular order, methods used in my research:
- Stable-isotope probing (SIP)
- Fluorescent in situ hybridisation (FISH)
- Quantitative-microautoradiography coupled to fluorescent in situ hybridisation (Q-MAR-FISH)
- Denaturing gradient gel electrophoresis (DGGE)
- Enzyme assays
- Analytical chemistry
- Inductively coupled plasma atomic emission spectroscopy (ICP-OES)
- Continuous culture (substrate-limited chemostats)
- Polyacrylamide gel electrophoresis of proteins (SDS-PAGE, Native PAGE, BN-PAGE, CN-PAGE and NuPAGE)
- Gas chromatography (GC)
- Thin-layer chromatography (TLC)
- Paper chromatography (it might be old-fashioned but it works!)
- Polymerase chain reaction (PCR)