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ePortfolio of Aditi Kibe

PhD candidate in Bioenergy and Industrial Biotechnology, MIBTP cohort 2016

Primary Supervisor: Prof. Nicholas Dale

Second Supervisor: Prof. David Roper

Project: Developing a novel biosensor for detection of neurochemicals in blood.

The field of biosensors has proven to be extremely innovative, especially in the area of biomedical applications. Recent advances in the field have allowed for biosensors to be developed as a self-contained analytical device that can measure analytes in the body fluids and allow rapid monitoring of physiological changes. The Dale lab has developed purine detecting biosensors for purines: ATP and adenosine, and other neurotransmitters such as acetylcholine and Glutamate. The lab has pioneered the development of enzymatic biosensors. We have proprietary methods and techniques for design of amperometric enzymatic biosensors.The aim of this project is to design an enzyme-based biosensor, that can detect other brain specific amino acids such as L-aspartate. The sensor would enable in detecting the levels of the amino acid in different parts of the brain and would shed light on the role it plays in neurotransmission. The project is funded by BBSRC.

Dale Lab

Prior Research Experience

  • Research Assistant, Warwick Medical School, University of Warwick (Nov 2015- Sept 2016)

Supervisor: Prof.Stephen J.Royle

• Worked on gene editing and generation of stable cell lines using the CRISPR/Cas9 technique.
• Managing and screening CRISPR clones using genomic DNA PCR

  • Research Techinician, School of Life Sciences , University of Warwick(March 2014- Oct 2015)

Supervisor: Prof. David J.Evans

Project: ‘Unravelling the impact of the mite Varroa destrcutor on the interaction between the honeybees and its

viruses'.

• Constructed plasmids for expression of viral proteins.
• Cross-absorbed and tested antibodies against deformed wing virus by Western blot.
• Larval feeding experiments in order to study RNAi response to the virus.
• Sequencing of viral cDNA clones, sequence assembly using DNAstar.
• Cloning and tagging of full length viral recombinants, larval test injections and analysis by quantitative RT-PCR, cloning and sequencing.

  • Techinician (Voluntary work), School Of Life Sciences, University of Warwick (Sept 2011- Nov 2011, Oct 2013)

Supervisor: Dr. Emma C.Anderson

• Assisted with mutagenesis and cloning of viral sequences for a project involving HIV-1 RNA

Education

MS : Cell and Molecular Biology, San Diego State University (2011)
MSc: Biochemistry, Devi Ahilya Vishwavidyalaya (2004)
BSc: Biology and Chemistry, Devi Ahilya Vishwavidyalaya (2002)