The identification and characterisation of proteins and protein complexes is central to the work carried out in my laboratory. As Director of the University of Warwick Proteomics Research Technology Platform my group uses cutting-edge analytical technologies in pursuing these goals. Our laboratory is equipped with a number of mass spectrometers including timsTOF Pro, Orbitrap Fusion (with ETD) and triple quadrupoles.

Protein phosphorylation site analysis is an area of increasing speciality within the Proteomics group, underpinning much of the research carried out by the many Cell Signalling groups at the University of Warwick. Strategies typically involve chromatographic separation followed by phosphopeptide enrichment using IMAC or TiO₂. The raw power of the Orbitrap Fusion is utilised for phospho site discovery and the specfic scan features (SRM) of the Triple Quadrupole are harnessed for validation.

Together with many colleagues from the School of Life Sciences and Warwick Medical School, I am currently working on a diverse range of proteomics projects.

Subtle changes in protein expression are increasingly key in many of these studies. Quantitative proteomic approaches, both labelled (Silac and iTRAQ) and label-free (DIA, SRM and Spectral Counting), are being utilised to elucidate these changes.