Services and Applications
Services and Application Areas
- Identification and Quantification of Proteins
Specialism in peptide-based proteomics to identify and quantify proteins from complex biological samples.
Samples can be provided as gel-bands, enriched via beads or extractions from cell lysates or tissue homogenates.
Co-immunoprecipitations, proximity labelling (BioID, Apex2, TurboID etc.), purified proteins, cross-linking, complex mixtures.
- Relative quantification via label-free (LFQ) or stable isotope labelling (iTRAQ, TMT or SILAC).
- Absolute quantification of proteins and metabolites using LC triple quadrupole selected reaction monitoring (SRM).
- Localisation of Post-translational protein modifications such as phosphorylation, acetylation or ubiquitination....
- Top-down Protein Analysis
Protein molecular weight determination by LC-MS. Non-covalent interactions. Ion mobility mass spectrometry.
- MALDI imagingLink opens in a new window of tissues or biofilms for biomolecule localisation.
- Discussion and advice on all aspects of experimental design, sample preparation, mass spectrometry stategies and data analysis.
