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Confocal Microscopy

Conventional optical microscopes display a shallow depth of field, especially at higher magnifications. A confocal microscope uses a pinhole to collect light from the focal plane of a sample, discarding out-of-focus light to produce a clear, high resolution image.

cogsHow does it work?

A laser is scanned across the sample, focused at a particular depth. Light from the sample passes through a small aperture (pinhole) which selects only light which contributes to an in-focus image.

Typically fluorescent species or tags are observed by selecting an appropriate wavelength of incident laser light and a suitable detection window. Multiple detectors permit several fluorophores to be monitored simultaneously.

Three-dimensional views can be obtained by stacking images from different heights in a sample, and fast scanning modes enable dynamic processes to be followed.

Applications:

Electrochemistry, neuroanatomy and neurophysiology; morphological studies of cells and tissues; resonance; energy transfer; stem cell research; photobleaching studies; lifetime imaging; multiphoton microscopy; total internal reflection; DNA hybridization; membrane and ion probes; bioluminescent proteins; epitope tagging.

Sample Handling Requirements:

Typically solid, with fluorophore(s).

Complementary Techniques:

Optical Microscopy, SEM.

Warwick capability:

Leica SP2 with 4 lasers, Leica SP5 with tunable white light laser.

Contact:

Dr Ian Hancox, 024 76 150380 email i dot hancox at warwick dot ac dot uk.

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Typical results:

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Status
Availability


Warwick collect/analyse data

Warwick collect data
 
Available to user with expertise/ contribution
 
Spare capacity for collaborative research
 

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