Fourier Transform Ion Cyclotron Resonance (FT-ICR)
Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR), also known as Fourier Transform Mass Spectrometry (FT-MS), is a type of mass analyzer (or mass spectrometer) for determining the mass-to-charge ratio (m/z) of ions based on the cyclotron frequency of the ions in a fixed magnetic field.
How does it work?
The key features of this type of mass spectrometry are extremely high resolving power (>1M, sufficient to resolve splittings 0.001 Da apart at m/z 1000) and high mass accuracy (<1 ppm routinely, <0.1 ppm with effort).
Sensitivities mostly depend on the ionization source, not the detector, but for MALDI and ESI, detection limits are in the mid-low femtomole range routinely, and low attomole range with effort (provided the sample is sufficiently clean – see below).
Protein sequencing; protein ID; protein PTM analysis; other biomolecule analysis; petroleomics; oil; metabolomics, etc.
Sample Handling Requirements:
Very clean samples are required. Ideally pure samples in a 10 mM ammonium acetate buffer.
Extensive instrumentation expertise. High mass accuracy and high resolution expertise. Particular experience in Proteins and Petroleum sample analysis, Bruker solariX 12.
Dr Ian Hancox, 024 76 150380 email i dot hancox at warwick dot ac dot uk.
Typical results format, and sample:
|Warwick collect/analyse data|
|Warwick collect data|
|Available to user with expertise/ contribution|
|Spare capacity for collaborative research|