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Fourier Transform Ion Cyclotron Resonance (FT-ICR)

Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR), also known as Fourier Transform Mass Spectrometry (FT-MS), is a type of mass analyzer (or mass spectrometer) for determining the mass-to-charge ratio (m/z) of ions based on the cyclotron frequency of the ions in a fixed magnetic field.

cogsHow does it work?

The key features of this type of mass spectrometry are extremely high resolving power (>1M, sufficient to resolve splittings 0.001 Da apart at m/z 1000) and high mass accuracy (<1 ppm routinely, <0.1 ppm with effort).

Sensitivities mostly depend on the ionization source, not the detector, but for MALDI and ESI, detection limits are in the mid-low femtomole range routinely, and low attomole range with effort (provided the sample is sufficiently clean – see below).

Applications:

Protein sequencing; protein ID; protein PTM analysis; other biomolecule analysis; petroleomics; oil; metabolomics, etc.

Sample Handling Requirements:

Very clean samples are required. Ideally pure samples in a 10 mM ammonium acetate buffer.

Complementary Techniques:

GC-MS, LC-MS.

Warwick capability:

Extensive instrumentation expertise. High mass accuracy and high resolution expertise. Particular experience in Proteins and Petroleum sample analysis, Bruker solariX 12.

Contact:

Dr Ian Hancox, 024 76 150380 email i dot hancox at warwick dot ac dot uk.

FTICR equipment

Typical results format, and sample:

FTICR sample

Status
Availability
green_tick.gif Warwick collect/analyse data
green_tick.gif Warwick collect data
 green_tick.gif Available to user with expertise/ contribution
 green_tick.gif Spare capacity for collaborative research
 

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