To enable the investigation of DNA by Next Generation Sequencing, the DNA starting material has to be processed to create a sequencing library. This is carried out using a number of molecular biology processes and, in this case, one of two library prep kits from Illumina with the Nextera, transposase technology.
Once a library has been made it can then go on to be sequenced on one of the Illumina sequencing platforms (see DNA Sequencing – MiSeq)
DNA libraries can be used to study whole genome sequence and structure, SNPs and other genetic variants as well as many aspects of the genome.
How does it work?
The Nextera and Nextera XT library preps begin by fragmenting the genomic DNA starting material and attaching known, adapter sequences to those DNA fragments. This is done using transposon insertion. The DNA adaptors are known DNA sequences that allow the library fragments to anneal to the sequencing flowcell. A sequencing run can then be performed.
Other DNA library prep protocols are available but are costed on a per project basis.
Each Nextera library requires 50ng of good quality DNA, each Nextera XT library (for small genomes) requires 1ng.
Dr Ian Hancox, 024 76 150 380
email i dot hancox at warwick dot ac dot uk.
Mrs Jeanette Selby, 024 76 5 75172
email j dot m dot selby at warwick dot ac dot uk.
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