Fourier Transform Ion Cyclotron Resonance (FT-ICR)
Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR), also known as Fourier Transform Mass Spectrometry (FT-MS), is a type of mass analyzer (or mass spectrometer) for determining the mass-to-charge ratio (m/z) of ions based on the cyclotron frequency of the ions in a fixed magnetic field.
How does it work?
The key features of this type of mass spectrometry are extremely high resolving power (>1M, sufficient to resolve splittings 0.001 Da apart at m/z 1000) and high mass accuracy (<1 ppm routinely, <0.1 ppm with effort).
Sensitivities mostly depend on the ionization source, not the detector, but for MALDI and ESI, detection limits are in the mid-low femtomole range routinely, and low attomole range with effort (provided the sample is sufficiently clean – see below).
Applications:
Protein sequencing; protein ID; protein PTM analysis; other biomolecule analysis; petroleomics; oil; metabolomics, etc.
Sample Handling Requirements:
Very clean samples are required. Ideally pure samples in a 10 mM ammonium acetate buffer.
Complementary Techniques:
Warwick capability:
Extensive instrumentation expertise. High mass accuracy and high resolution expertise. Particular experience in Proteins and Petroleum sample analysis, Bruker solariX 12.
Contact:
Claire Gerard: / 07385 145064