Liquid Chromatography Mass spectrometry (LC-MS, or alternatively HPLC-MS) is an analytical technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry.
How does it work?LC-MS is a powerful technique used for many applications which has very high sensitivity and selectivity. Generally its application is oriented towards the specific detection and potential identificationof chemicals, metabolites, peptides and proteins in a complex mixture.
Detection of selected molecules, quantitative proteomics, metabolism studies, environmental detection, trace analysis. Often coupled with tandem mass spectrometry to obtain (via multiple reaction monitoring (MRM)) additional selectivity and improved signal to noise.
Sample Handling Requirements:
Micrograms or nanograms of material.
GC-MS, size exclusion chromatography-MS.
Extensive instrumental resource with Orbitrap Fusion, Quantiva triple quadrupole, quadrupole-time-of-flight, TOF-TOF and FT-ICR capability, Bruker MaXis, Waters Synapt G1, Acquity Xevo-TQ, Acquity TQD
Dr Ian Hancox, 024 76 150380 email i dot hancox at warwick dot ac dot uk.
Typical results format, and sample:
|Warwick collect/analyse data|
|Warwick collect data|
|Available to user with expertise/ contribution|
||Spare capacity for collaborative research|