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Growing Away From Trouble in a Challenging Climate – Validation and Trait Integration of CRISPR-Modified Rapid Rooting Lettuce
Secondary Supervisor(s): Professor Miriam Gifford, Dr Andrew Beacham
University of Registration: Harper Adams University
BBSRC Research Themes:
Project Outline
Breeding crops which are resilient to climate change is becoming increasingly essential, and plants that quickly establish root systems have the potential for faster establishment, improved tolerance to abiotic stresses and decreased reliance on inputs such as fertilisers (Roberts et al. Annals of Applied Biology 2020). Hence, root traits are a key area of interest in respect to sustainable intensification of production systems.
This project investigates newly isolated CRISPR rapid rooting lines in lettuce (Lactuca sativa) that originate from previous PhD research carried out in collaboration between the supervisory team.
The project has three aims which can be pursued in parallel, maximising the chance of student success:
- Characterisation of rapid rooting lettuce lines, using plant genotypes that are available from a recent PhD project. This will involve confirming CRISPR edits then carrying out preliminary phenotypic and molecular analysis to characterise the mechanism of beneficial effects on lettuce growth and yield.
- Phenotypic analysis of rapid rooting lettuce lines in greenhouse and commercial growing conditions (including hydroponics) in order to document full-cycle performance in hydroponic systems used by growers.
- Determine the impact of rapid rooting on other traits including drought resilience and nutrient stress conditions. Bioassays that lead to detrimental stress effects in wild-type plants will be run for rapid rooting lines to test for additional beneficial effects.
The project benefits from having access to both the excellent crop research facilities and growth techniques (Wharton et al. Plant Methods 2025) and links to industry partners at Harper Adams University, as well as the bioinformatics and molecular expertise at the University of Warwick.
Overall, the work will validate the agronomic benefits of affecting rapid rooting expression on lettuce yield, and assess impacts on drought tolerance and nutrient uptake.