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Upcycling Farm Residues into Fungal Bio-inks for 3D Printing
Secondary Supervisor(s): Professor Gary Bending
University of Registration: University of Warwick
BBSRC Strategic Research Priority:
Project Outline
This research plan is a collaboration between the School of Life Sciences and the School of Engineering, which will see the candidate work on fundamental aspects of biomaterials development while at the same time leading to practical applications of 3D bio-printing.
This project focuses on fungal fermentation processes as a potentially viable source for the development of sustainable ‘bio-inks’ which can then be used for the 3D printing of environmentally friendly structures. Fungi, including filamentous fungi and yeast, have shown the potential to be used as alternative biomaterials due to their ability to secrete extracellular enzymes and metabolise a diverse range of substrates for a wide range of applications including construction and insulation elements. Specifically, we aim to explore ‘Mycelium’ (the vegetative part of fungi) and its potential ability to replicate the shape and function of close-cell foams and synthetic fibres. This possibility has been suggested by recent studies with mycelium being used as a structural scaffold in combination with agricultural waste, such as corn stover or rice husks, to create biodegradable foam-like materials with desirable mechanical properties.
We intend to harness the current progress in bio-fabrication technology to apply state-of-the-art 3D-Printing for the precise custom-deposition of the resulting complex organic slurry formulations. This will enable the research team to create and design complex compliant structures whose behaviour can replicate the performance observed in synthetic materials (i.e., glass wool, stone wool, polystyrene, etc).
For the fundamental laboratory-based research, we will create a range of Mycelium-based bio-composites based on our experimental design, which will include: research and selection of potentially viable fungal strains, experimental design and selection of suitable growth and proliferation media for cultures. A range of agricultural waste bioproducts will be studied on their capacity to act as rich substrate which provides sufficient nutrients to promote fungal growth. Previous literature suggests agro-industrial residues such as corn starch, potato starch or sugar cane wet dust, to be viable substrates for solid-state fermentation; however the current processes are variable and highly dependent on the local conditions, climate, humidity and diversity, therefore we seek to develop a process that is appropriate for UK conditions and in particular that benefits from local agricultural-waste from the West-Midlands.
Different viable cultures will be created and transferred to a substrate to investigate colonization performance and seeding strategies. Our goal is to develop a range of Mycelium-based bio composites whose mechanical behaviour can be tuned according to the application, allowing us to create a range of bio-inks with pre-defined characteristics such as ‘stretchy-flexible’ or ‘hard-tough’.
In summary, the core activities of the project will include:
On the biologic/wet-lab aspect:
- Explore mycelium cultures, media, nutrients, growth rates and ‘survival’ when mixed into ink/slurries.
- Control growth parameters to obtain different densities/consistencies (density, flexibility, hardness, etc.).
- Develop repeatable and viable bio-inks which can be consistently used for dispensing.
On the engineering aspect:
- Characterize the rheologic and flow behaviour of the various ink formulations.
- Characterize sample parts based on 3 main parameters: extrudability, flowability, and shape stability (similar to what we do for concrete).
- Mechanical characterization: hardness, compression, flex tests of coupons.
As our ultimate goal, we expect the use of nature-derived fungal fibrous materials will have the potential to improve the current waste management and to drastically reduce CO² emissions mainly at the material creation and processing stages.
References
Enriquez-Medina et.al (2024) ‘Bridging gap between agro-industrial waste, biodiversity and mycelium-based biocomposites’ Journal of Bioresources and Bioproducts, https://doi.org/10.1016/j.jobab.2024.07.001.
Gantenbein, S., Colucci, E., Käch, J. et al. Three-dimensional printing of mycelium hydrogels into living complex materials. Nat. Mater. 22, 128–134 (2023). https://doi.org/10.1038/s41563-022-01429-5.