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Time, Trees, and Disease: Exploring how metabolic variation in cherry influences Pseudomonas syringae pathogens
Secondary Supervisor(s): Dr Diana Vinchira-Villarraga
University of Registration: University of Birmingham
BBSRC Research Themes:
Project Outline
Bacterial canker represents one of the most damaging diseases of cherry trees (Prunus spp.). The causal agents of this disease are specific pathogenic varieties of Pseudomonas syringae (Pss and Psm) that infect leaves, wood, buds, and fruits. Symptoms on leaves and fruits are observed during summer, whilst cankers on wood are evident at the end of tree dormancy. In the UK, Pss and Psm show variable distribution across cultivated and wild cherries, with different strains exhibiting varying pathogenicity, which is associated with the presence of effector genes and/or toxin biosynthetic gene clusters that contribute to Pss and Psm fitness and pathogenicity in a tissue- and host-dependent manner. The diversity in symptom development, the differential population of Pseudomonas strains across plants, and the disease cycle itself are indicative of a finely tuned interaction between the pathogen's biology and the host's phenology. Such interaction may be influenced by seasonal changes in plant metabolism, which occur to support growth and developmental transitions. The availability of specific nutrients may affect Pss/Psm growth, metabolism, and gene expression, ultimately impacting disease development. However, these changes have not been evaluated in detail yet in the Pss/Psm-cherry pathosystem. It is still not well understood how the metabolic profiles of ornamental and wild cherry differ, and whether this impacts the Pseudomonas population. Additionally, there is limited information on how the chemical profile of cherry varies across phenological stages. This PhD project will address these gaps by integrating plant and bacterial untargeted metabolomics, transcriptomics, and plant disease phenotyping. Through a combination of field sampling, controlled glasshouse experiments, in vitro assays, and omics approaches, the project will provide new insights into the role of the cherry metabolic interface in shaping Pss/Psm behaviour and influencing bacterial canker development.