Dr Wolfram Gruhn
Supervisor Details
Research Interests
The epigenome is a critical regulatory layer for DNA transcription, playing an essential role in defining the identity and developmental potential of cells. In mammals, cells of the early embryo are totipotent, signifying their ability to develop into any cell type formed in an individual's lifetime. A key foundation for totipotency and normal embryo development is laid in the embryonic germline, when primordial germ cells (PGCs) erase a substantial part of their epigenetic memory, in a process termed epigenetic resetting.
My research focuses on understanding the molecular mechanisms that govern the development of human PGCs and the intricate process of epigenetic resetting. Here, my lab aims to address: 1.) What transcription factor networks drive human PGC development? 2.) How do human PGCs decide which epigenetic information to erase or maintain? 3.) Can factors associated with the maternal environment or diseases impact human PGC development?
To address these questions, my lab uses human embryonic stem cell-based model systems to recapitulate early germline development in combination with various approaches for monitoring and manipulating transcription and protein-DNA interactions including RNA-seq, CUT&RUN, inducible degrons, and CRISPR.
Ultimately, the research in my lab aims to identify risk factors for infertility and abnormal development of the germline.
Research Groups
MIBTP Project Details
Current Projects (2025-26)
Primary supervisor for: