This page is under continuous construction. If you find bugs or would like to suggest improvements email j dot r dot lewandowski at warwick dot ac dot uk.
Disclaimer: All of the pulse programs and macros were tested on our 600 MHz Bruker Avance II+ or 850 MHz Bruker Avance III spectrometers. They should be generally free of any major errors but use them at your own risk.
Bruker Pulse Programs
13C' and 15N R1 and R1ρ relaxation rates measurements (based on S3E-DCP)
Pulse sequences for measuring 13C' and 15N R1 and R1ρ using an experiment based on DCP with S3E block to remove splitting due to 1JCC (Lamley et al. PCCP DOI: 10.1039/C5CP03484A) will posted here soon. Until then contact the authors.
Bruker Decoupling Programs
Swept Low Power TPPM
Sequence: Swept Low Power TPPM (slpTPPM), example: sltppm_40pTr41
Set up: 1. Set the pcpd to 2x rotor period, 2. The amplitude for the 1H field should be close to 1/4 of the spinning frequency. Ideally one should optimize decoupling strength over echo.
What is it useful for: Heteronuclear decoupling at spinning frequencies > 40 kHz in fully protonated systems. We also use it for solvent saturation in proton detected experiments (usually 50-200 ms).
If you use it, please cite: Lewandowski, J.R. et al. J. Am. Chem. Soc. 132, 8252-4 (2010).
caltemp - Calculate approximate temperature of your protein based on the chemical shift of water. Assumes use of the DSS (4,4-Dimethyl-4-silapentane-1-sulfonic acid) as the internal reference. The macro takes into account the pH and salt concentration. For the original references see: Hoogen, Y. T. van den, et al. (1988) Eur. J. Biochem., 171, 155-162 (original formula) and Wishart, D. S.,et al. (1995) J. Biomol. NMR, 6, 135-140 (dependence of water chemical shift on the pH and salt concentration).