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Chemistry Lewandowski Group

Lewandowski Group

Lewandowski group focuses on development and applications of solid-state and solution NMR to study structure, dynamics and interactions of biological systems in the context of integrated structural biology. We are associated with the Warwick solid-state NMR grouping and Chemical Biology Research Facility.

Solid-State NMR Methodology

We develop methods to probe structure and dynamics of molecules.

Protein structure & interactions

We apply both solid- and solution-state NMR to various biomolecular systems.

Natural products biosynthesis

Structural biology and engineering of natural products biosynthetic machinery.

Protein dynamics

Experimental determination and simulation of molecular motion.

Antibiotics

Understanding modes of action and facilitating designing new antibiotics.

News

   
MAS rotors versus maximum spinning frequency and volume (click for a larger image)
Solid-State NMR Methodology Development

We develop solid-state NMR methods for studying structures and dynamics of proteins.

Highlights:

  • NMR relaxation and methodology at 60-111 kHz magic angle spinning (PCCP 2015).
  • Characterisation of structure and dynamics of large protein complexes (JACS 2014, Angew. Chem. 2015)
  • Solvent PREs for characterisation of protein-protein interfaces in large complexes (JACS 2017)
  • Accelerated R relaxation dispersion for application in large complexes (SciRep 2019)
gb1igg-complex-17march2014v2.png
Protein structure determination and molecular interactions

We employ a combination of different structural biology and biophysical methods led by solid-state and solution-state NMR to investigate structure, dynamics and interactions of various biological systems. We work on systems that are natural targets for solid-state NMR, e.g. nanocrystalline proteins, protein complexes (e.g. protein-antibody complexes and complexes from polyketide synthases), fibrils and membrane proteins and systems that benefit from combined solution/solid state NMR approaches. We are also interested in characterization of molecular interactions, in particular protein interactions with other proteins, ligands and nucleic acids.

Main areas:

3D GAF
Protein dynamics

We apply a variety of methods centered around NMR to characterize protein motions at atomic resolution.

Highlights:

  • dynamics of GB1 in a >300 kDa complex with full length immunoglobulin (Angew. Chem. 2015)
  • protein "dynamic transitions" through the lens of variable temperature relaxation measurements in a crystalline protein (Science 2015)
  • peptide plane fluctuations from joint 15N and 13C' relaxation measurements (PhysChemChemPhys 2015)
 Bamb_5917 PCP:Bamb_5915 C complex model
Structural biology and engineering of systems involved in natural products biosynthesis

We employ NMR-led integrated structural biology approach to study mechanistic details of various PKS/NRPS biosythetic pathways.

Highlights:

  • Structural basis for chain release from the enacyloxin polyketide synthase. Integrated structural biology approach (Nat. Chem. 2019)
  • Mechanism of intersubunit ketosynthase–dehydratase interaction in polyketide synthases (Nat. Chem. Biol. 2018)
  • Review on the protein-protein interactions in trans-AT PKSs (Nat. Prod. Rep. 2018)

NMR facilities

(NMR hall in Millburn House)

The group uses mostly 500 MHz (various MAS probes from 4 mm to 1.3 mm), 600 MHz (various MAS probes from 4 mm to 1.3 mm, a unique 0.8 mm 100 kHz probe from Ago Samoson). We are also frequent users of the National 850 MHz Solid-State NMR Facility.

Millburn House NMR Hall

(NMR hall in MAS building - left to right: solid-state NMR: 700 MHz WB spectrometer with 0.7, 1.3 and 3.2 mm MAS probes, solution NMR: 500 MHz, 600 MHz, 700 MHz with cryoprobe).

The group is the main user of the ERC & UW funded 700 MHz solid-state NMR spectrometer and frequent users of 700 MHz and 600 MHz solution NMR spectrometers.

NMR Hall in MAS building