The main aim was to identify the diffusion coeficient from the FRAP data, which is important for the study of the mobility characteristics of the molecules. This work was based on a bigger research project which aimed in showing whether there is a diffusion barrier during potrusion of any cell. This barrier could act as a trapping mechanism of proteins necessary for the polymerization of actin filaments.
Aureins are an important class of antibiotic peptides which show antibacterial and anticancer properties and are able to interact with cell membranes (Figure below). The aim of this project was to investigate various factors which affect the preference of those peptides towards bacterial membranes and gain an insight as to how the peptides manage to lyse the membrane and consequently cause cell death. These interactions were studied via Linear Dichroism (LD) and Circular Dichroism (CD) across a different types of liposomal formulations.
Protein Disulfide Isomerase is an enzyme responsible for catalyzing the formation of disulfide bonds in secretory proteins. The crystallization of this enzyme is extremely difficult due to its flexibility and the rapid dynamics that govern its domains. For this reason, FRET (Fluorescence Resonance Energy Transfer) is being looked forward to be used as a tool to provide information about intramolecular distances. The fluorescent labelling of this molecule at certain domains is necessary for this technique to be carried out, which entailed the main aim of this project. The expression and purification of this protein was necessary prior to the development of the labelling protocol.