About My Project
Secondary structure, and consequently the folding state of a protein, plays a major role in its function as it is the key element in the prediction of tertiary structure and also useful in assessing protein aggregation and stability. The use and combination of several spectroscopic techniques will serve for the purpose of estimating the protein motif of various biopharmaceutical proteins.
Highly sensitive optical measurements, such as CD (circular dichroism) (Fig. 1), IR and Raman spectroscopies are quite rapid and require very little material, thus contributing to the concept of Quality by Design. In addition to this, high-resolution 1H-NMR (Fig. 2) spectroscopy can provide us with a rough idea about the secondary structure and the stability of a protein, while 2D and nD NMR provides more detailed information about the structure and dynamics of proteins at the atomic level. The combination of NMR with the previously mentioned low-resolution methods will help to bridge the gap between amino acid sequence and function by providing clues to the structure a protein may adopt.
Figure 1: CD spectra of insulin at increasing Temperature.
Figure 2: 1H-NMR spectra of insulin at increasing Temperature.
2013 - Present:
Protein biopharmaceuticals: probing correct folding with new near IR, polarized Raman spectroscopy, circular dichroism, and NMR spectroscopies
- PhD (Ongoing), Analytical Science, MOAC DTC, University of Warwick
Supervisors: Prof. Alison Rodger (Warwick), Dr. Claudia Blindauer (Warwick), Dr. Ian Sanders (Bruker Daltronik GmbH)
2009 - 2012:
- M.Sc. in Synthetic Pharmaceutical Chemistry, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens (Greece)The Design and Synthesis of New Aminosubstituted Pyrazolo[3,4-c]pyridines as Potential Kinase InhibitorsSupervisor: Prof. Panagiotis Marakos (NKUA)
2004 - 2009:
- B.Sc., Faculty of Pharmacy, National and Kapodistrian University of Athens (Greece)Diploma Thesis: Synthesis and Tautomerism Study of 5-substituted pyrazolo[3,4-c]pyridines
Supervisor: Prof. Panagiotis Marakos (NKUA)
Top Floor, Senate House
University of Warwick
Tel: 024 765 75808
Chemistry Department, B608
University of Warwick
Tel: 024 765 23293
Email: M dot Sklepari at warwick dot ac dot uk