Application Areas:

• Top-down Protein Analysis
• Large Biomolecules Analysis
• Protein and Peptide Sequencing
• Post-Translational Modification Identification and Localisation
• Conformer and Isomer Analysis
• Lipidomics Analysis
• DNA & RNA Analysis
• Polymer Identification and Structural Analysis
• Metabolomics Analysis and Structural Characterisation
• Petroleomics Analysis
• Environmental Studies

Routine Experiments that the Advance Mass Spectrometry RTP can offer are as follows:

1. High resolution and high mass accuracy mass analysis - limited to ions from m/z 150 – 2500 - limited to masses up to 40 kDa - limited to the following solvent systems (H2O, MeCN, MeOH, EtOH, chloroform, dichloromethylene, IPA, toluene, hexane, acetone), but other solvents may work mostly depending on the purity of the solvent. - dual-spray internal calibration for guaranteed and traceable low-ppm to sub-ppm mass accuracy
   • Typical resolving power on FTICR-MS at 200 Da is > 1 million
   • Typical mass accuracy on FTICR-MS at 200 Da is < 0.1 ppm
   • Typical resolving power on FTICR-MS at 1000 Da is > 500,000
   • Typical mass accuracy on FTICR-MS at 1000 Da is < 0.3 ppm
2. MS/MS of various molecular species
   • Collision-induced dissociation (CID)
   • Infrared multiphoton dissociation (IRMPD) (10 micron)
   • Ultraviolet photodissociation (UVPD) (213 nm, potentially 266 nm)
   • Electron capture dissociation (ECD) (Require multiply-charged positive ions)
   • Electron impact dissociation (EID)
   • Electron detachment dissociation (EDD) (negative ions)
   • Electron transfer dissociation (ETD)
   • Data dependent acquisition modes (DDA)
   • Data Independent Analysis (DIA)
   • Two-dimensional Mass Spectrometry (2DMS)
3. Ion mobility mass spectrometry using trapped ion mobility spectrometry (TIMS) - can be combined with collision induced dissociation - possibly collision cross section measurement, with appropriate calibration
4. Electrospray ionization (ESI) and nanoESI
5. Matrix-assisted laser desorption/ionization (MALDI)
6. Atmospheric pressure photoionization (APPI) and atmospheric pressure chemical ionization (APCI)
7. Nanomate automated runs (96 samples at a time, but with a £500 extra consumables charge)
8. Online LC-MS or GC-MS
9. Offline fractionation using HPLC

Please contact us to discuss your samples.