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Media Preparation Service

Reducing the burden of preparing routine and large volumes of media

Media Prep The School of Life Sciences, University of Warwick benefits from having its own in-house Media Preparation Service.

The primary aim of the service is to reduce the researcher’s burden of preparing routine and large volumes of media. Making significant time and financial savings, this allows research groups and lab personnel to dedicate more valuable time to research and gathering data.

To place an order for media please use the online order form

The service also supports our Wellesbourne Campus, Warwick Crop Centre and Warwick Medical School. In an average year the team pours in excess of 50,000 agar plates and produces over 12,000 litres of media. This includes 4,500 litres of bacteriological and yeast media, 6,000 litres of buffers, plus a wide variety of cell culture, antibiotics and associated products. In addition to this over 1,000 bespoke/customised orders are fulfilled per annum.

Product range

An extensive range of commonly used buffers, bacteriological and yeast media, cell culture, antibiotics, amino acid solutions and associated products are routinely prepared and are available as stock items. If products/solutions you or your lab use frequently are not listed in our catalogue (available from the Media Prep Room), please contact us to discuss potential additions to our range.

Sterile filtration

When autoclaving is not the preferred method of sterilisation, the Media Preparation Lab has facilities to filter sterilise large volumes of media, using a positive-pressure filtration system. The service can filter sterilise up to 20 litres of media at a time, in aliquots ranging from 10, 20, 100, 200, 400 and 800 ml routinely. If you require different sized aliquots, this can easily be accommodated.

Plate pouring

Automated plate pouring equipment allows the production of up to 600 standard 90 mm agar plates per hour. A range of commonly used plates are always available as stock items. Bespoke and custom prepared plates can be manufactured upon request, at varying thicknesses, if required. Note: when requesting plates, we generally pour in multiples of 30. We also have facilities to pour 60 mm petri dishes. Alternatively, agar can be prepared and kept molten for you to pour different sized plates yourself.

Turn around times

The service aims to complete bespoke orders as quickly as possible: solutions usually within 2-3 days; poured plates usually within 5 days. Sometimes your order may take a little longer due to workload and acquisition of necessary components. Estimated completion dates are given at the point of ordering. If you have a genuinely urgent request, we will always endeavour to assist.

Water

The preparation of all products (unless otherwise requested) begins with UV treated 0.2µm filtered high purity, type 1 pharmaceutical grade 18 MΩ-cm Super-Q water.

Quality control

The Media Preparation Service has an on-going commitment to producing high-quality and trusted products, with all media being fully traceable. This is supported by regular checks and calibration of equipment used within the facility. This includes periodic external validation of autoclaves, balances and pipettors. In addition:
  • Filter sterilised products
    Prior to use, multiple samples from each batch are combined with bacteriological broths and incubated to confirm the sample is free from aerobic and anaerobic bacteria, yeast and fungal contamination. After a 10 day test-period, media is passed and released for use.
  • Autoclaved products
    Sterility of autoclaved media and buffers is confirmed by the use of biological indicators during the autoclaving process.

Customised media

Over 300+ types of media are currently produced annually. At times, you may require something a little different. The Media Preparation Team have the experience and expertise of creating custom-made media from scratch. This allows modifications to recipes and customisation to your exact needs. Often, this is not an option when commercially commissioned, as it is cost and time prohibitive. Contact us to discuss your requirements.

Media locations

  • The Media Preparation Service is based at our Gibbet Hill Campus, Room M021 (near to the School of Life Sciences Reception, adjacent to the Atrium).
  • Buffers and solutions stored at ambient temperatures e.g. PBS, TBE x 5, TBS x 10, TBS/Tween x 10, TAE x 50 and Tris/HCL are located on the First floor of the BMRI building, on shelves to the rear of Work Room M101.
  • Bacteriological and Cell Culture media is located in Cold Room MO20, adjacent to the Atrium and opposite to the Media Preparation Lab M021.
  • Antibiotics and Cell Culture products are stored in a Freezer Room, located to the rear of Cold Room M020.

Contact Us
We are always happy to discuss individual or lab requirements and any queries you may have concerning our products.

Working hours:
Mon – Thu 08:00 – 16:00 hrs
Fri 08:00 – 15:30 hrs
Tel: 02476 574240

Cerith Harries
Cerith Harries
Cerith dot Harries at warwick dot ac dot uk

Caroline Farrell

Caroline Stewart
C dot Farrell at warwick dot ac dot uk

Feedback
We value, welcome and encourage feedback. Constructive comments are always appreciated. If you think there are areas where our product range, service delivery or standards could be improved, please let us know.


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