TM data
Sheet1
DNA |
DNA+Fe2L3 |
Melting curves in 10 mM NaCl and 1 mM sod. cacod. | |||
t |
Abs |
t |
Abs |
DNA:Fe2L3 10:1 | |
20.01 |
0.9999996 |
20.02 |
1 |
||
20.5 |
1.000004 |
20.56 |
0.9996928 |
||
21 |
0.9999106 |
21.06 |
0.9997333 |
||
21.5 |
0.9994969 |
21.54 |
0.9997312 |
||
22 |
0.9999102 |
22.05 |
0.9997545 |
||
22.51 |
1.000054 |
22.54 |
0.999759 |
||
23 |
1.000098 |
23.05 |
0.9997571 |
||
23.5 |
1.000026 |
23.56 |
0.9997513 |
||
24.01 |
0.9998956 |
24.06 |
1.00003 |
||
24.5 |
0.9997882 |
24.54 |
0.9994319 |
||
25 |
0.9999 |
25.04 |
0.9997324 |
||
25.51 |
0.9997175 |
25.56 |
0.9997304 |
||
26 |
0.9996541 |
26.03 |
0.9997288 |
||
26.51 |
0.9999139 |
26.55 |
0.9997183 |
||
27 |
0.9995358 |
27.06 |
0.9994183 |
||
27.5 |
0.9994928 |
27.54 |
0.9997816 |
||
28.01 |
0.9986202 |
28.06 |
1 |
||
28.5 |
0.9986484 |
28.55 |
0.9996928 |
||
29 |
0.9986394 |
29.04 |
0.9997333 |
||
29.5 |
0.9985799 |
29.55 |
0.9997312 |
||
30.01 |
0.9985648 |
30.05 |
0.9997545 |
||
30.5 |
0.998608 |
30.55 |
0.999759 |
||
31 |
0.9986449 |
31.04 |
0.9997571 |
||
31.5 |
0.9986277 |
31.54 |
0.9997513 |
||
32 |
0.998648 |
32.03 |
1.001084 |
||
32.49 |
0.9986966 |
32.56 |
1.001024 |
||
32.99 |
0.9986941 |
33.04 |
0.9997324 |
||
33.5 |
0.9987001 |
33.53 |
0.9997304 |
||
33.99 |
0.9972383 |
34.06 |
0.9997288 |
||
34.5 |
0.997252 |
34.54 |
0.9997183 |
||
34.99 |
0.9972357 |
35.04 |
0.9998024 |
||
35.5 |
0.9972121 |
35.54 |
0.9998062 |
||
36.01 |
0.9972423 |
36.03 |
0.9997955 |
||
36.49 |
0.99727 |
36.55 |
0.9997931 |
||
37 |
0.9972694 |
37.03 |
0.9998123 |
||
37.49 |
0.9972277 |
37.54 |
0.9998028 |
||
37.99 |
0.9972293 |
38.04 |
0.9998047 |
||
38.5 |
0.9972293 |
38.53 |
0.9998096 |
||
38.99 |
0.9972551 |
39.05 |
0.9997813 |
||
39.5 |
0.9972071 |
39.54 |
0.9997848 |
||
39.99 |
0.9972059 |
40.04 |
0.9997827 |
||
40.49 |
0.9972008 |
40.54 |
0.9997655 |
||
41 |
0.997204 |
41.03 |
0.9997752 |
||
41.5 |
0.9972 |
41.54 |
0.9997886 |
||
42 |
0.9972166 |
42.05 |
0.9997813 |
||
42.5 |
0.9972061 |
42.52 |
0.9997621 |
||
43 |
0.9972222 |
43.04 |
0.9997521 |
||
43.5 |
0.9972104 |
43.56 |
0.9997621 |
||
43.99 |
0.9967001 |
44.03 |
0.9997577 |
||
44.5 |
0.9970288 |
44.55 |
0.99977 |
||
44.99 |
0.9961742 |
45.02 |
0.9997624 |
||
45.49 |
0.9960612 |
45.54 |
0.9997486 |
||
46 |
0.9967779 |
46.04 |
0.9990879 |
||
46.5 |
0.9952921 |
46.54 |
0.9990478 |
||
47 |
0.9963658 |
47.04 |
0.9990829 |
||
47.49 |
0.9963443 |
47.55 |
0.9991047 |
||
47.99 |
0.9953181 |
48.05 |
0.9990505 |
||
48.5 |
0.9964243 |
48.54 |
0.9988356 |
||
49 |
0.9960676 |
49.03 |
0.9989048 |
||
49.5 |
0.9949332 |
49.54 |
0.9987792 |
||
50 |
0.9953164 |
50.04 |
0.9986833 |
||
50.49 |
0.9954103 |
50.53 |
0.9990207 |
||
50.99 |
0.9950936 |
51.05 |
0.9987361 |
||
51.5 |
0.9951104 |
51.54 |
0.9988883 |
||
51.99 |
0.99467 |
52.04 |
0.9990551 |
||
52.5 |
0.9945261 |
52.56 |
0.9989389 |
||
53 |
0.9943638 |
53.06 |
0.9989106 |
||
53.49 |
0.9940106 |
53.54 |
0.9990246 |
||
54 |
0.9937617 |
54.02 |
0.9987494 |
||
54.5 |
0.993961 |
54.54 |
0.9988099 |
||
55 |
0.9935622 |
55.04 |
0.9985712 |
||
55.5 |
0.9936892 |
55.56 |
0.9985852 |
||
55.99 |
0.9933214 |
56.04 |
0.9985189 |
||
56.5 |
0.9936264 |
56.54 |
0.9983789 |
||
56.99 |
0.9933057 |
57.06 |
0.9983632 |
||
57.49 |
0.9942311 |
57.54 |
0.9982402 |
||
58.01 |
0.9944937 |
58.04 |
0.9981536 |
||
58.5 |
0.994696 |
58.55 |
0.9980252 |
||
58.99 |
0.9960797 |
59.03 |
0.9980925 |
||
59.5 |
0.9968229 |
59.54 |
0.9979373 |
||
60 |
0.9989562 |
60.04 |
0.997836 |
||
60.5 |
1.001514 |
60.55 |
0.9976929 |
||
61 |
1.00526 |
61.06 |
0.9976409 |
||
61.5 |
1.010095 |
61.54 |
0.9984636 |
||
62 |
1.016456 |
62.03 |
0.9981809 |
||
62.49 |
1.023842 |
62.54 |
0.9989688 |
||
62.99 |
1.032167 |
63.06 |
0.9987051 |
||
63.5 |
1.041402 |
63.54 |
0.9984803 |
||
64 |
1.050492 |
64.06 |
0.9976122 |
||
64.5 |
1.05959 |
64.55 |
0.9969458 |
||
65 |
1.070124 |
65.07 |
0.9958135 |
||
65.51 |
1.080212 |
65.54 |
0.9945064 |
||
66 |
1.090554 |
66.05 |
0.9953344 |
||
66.51 |
1.101817 |
66.55 |
0.9967188 |
||
67 |
1.113211 |
67.05 |
0.9968058 |
||
67.5 |
1.123822 |
67.54 |
0.9980826 |
||
68 |
1.13345 |
68.06 |
0.9989784 |
||
68.5 |
1.143018 |
68.55 |
1.003228 |
||
69 |
1.151395 |
69.05 |
1.00718 |
||
69.5 |
1.15875 |
69.56 |
1.012539 |
||
70.01 |
1.165885 |
70.05 |
1.019731 |
||
70.51 |
1.172257 |
70.56 |
1.026499 |
||
71.01 |
1.178556 |
71.06 |
1.034986 |
||
71.5 |
1.185341 |
71.52 |
1.043099 |
||
72 |
1.192372 |
72.03 |
1.05224 |
||
72.5 |
1.197937 |
72.55 |
1.063246 |
||
73 |
1.201774 |
73.06 |
1.073105 |
||
73.5 |
1.204958 |
73.54 |
1.082173 |
||
74.01 |
1.2079 |
74.05 |
1.09267 |
||
74.5 |
1.211636 |
74.56 |
1.102552 |
||
75.02 |
1.216137 |
75.05 |
1.112242 |
||
75.5 |
1.220099 |
75.55 |
1.12009 |
||
76 |
1.224339 |
76.06 |
1.12721 |
||
76.5 |
1.227497 |
76.54 |
1.136176 |
||
77 |
1.229456 |
77.05 |
1.145406 |
||
77.52 |
1.230443 |
77.55 |
1.152498 |
||
78 |
1.231328 |
78.04 |
1.160493 |
||
78.5 |
1.233052 |
78.54 |
1.1674 |
||
79 |
1.234564 |
79.04 |
1.173436 |
||
79.5 |
1.236214 |
79.56 |
1.178892 |
||
80 |
1.238064 |
80.04 |
1.185298 |
||
80.5 |
1.239728 |
80.55 |
1.190727 |
||
81.01 |
1.241068 |
81.05 |
1.194909 |
||
81.51 |
1.242304 |
81.54 |
1.199369 |
||
82.01 |
1.243272 |
82.04 |
1.203449 |
||
82.5 |
1.244042 |
82.56 |
1.206263 |
||
83 |
1.244898 |
83.07 |
1.210174 |
||
83.5 |
1.245832 |
83.55 |
1.213446 |
||
84.02 |
1.24652 |
84.04 |
1.21567 |
||
84.51 |
1.246483 |
84.55 |
1.217792 |
||
85.01 |
1.247066 |
85.05 |
1.219938 |
||
85.51 |
1.247132 |
85.55 |
1.22312 |
||
86.02 |
1.247199 |
86.06 |
1.225445 |
||
86.5 |
1.247755 |
86.54 |
1.226686 |
||
87 |
1.247798 |
87.04 |
1.229053 |
||
87.52 |
1.247629 |
87.54 |
1.231292 |
||
88 |
1.247711 |
88.06 |
1.233143 |
||
88.5 |
1.248172 |
88.56 |
1.234811 |
||
89 |
1.24834 |
89.04 |
1.23565 |
||
89.5 |
1.248286 |
89.55 |
1.237188 |
||
90.01 |
1.248465 |
90.05 |
1.239808 |
||
90.5 |
1.2484 |
90.55 |
1.241093 |
||
91 |
1.24847 |
91.06 |
1.242692 |
||
91.51 |
1.248645 |
91.55 |
1.244178 |
||
92 |
1.248948 |
92.06 |
1.244951 |
||
92.5 |
1.249278 |
92.56 |
1.246133 |
||
93 |
1.249342 |
93.06 |
1.248828 |
||
93.51 |
1.249532 |
93.55 |
1.250618 |
||
94 |
1.249421 |
94.04 |
1.251419 |
||
94.51 |
1.249557 |
94.55 |
1.252342 |
||
95 |
1.249776 |
95.04 |
1.254328 |