My PhD is a semi-continuation of my first miniproject tracking kinetochores attached to chromosomes in mitotic cells. I will be using the same image analysis program making my own major modifications to allow multi-channel tracking of spindle pole positions as well as inner and outer kinetochore plate position. I will in parallel to this be developing a triple-stable HeLa cell line expressing fluorescent markers for the inner and outer kinetochore plates as well as the spindle poles. I will then be analysing the results and building physical/statistical mathematical models of the chromosome oscillations.
More details and images/movies to follow soon.
Video showing Hela cells with tagged Eb3 - a microtubule plus-tip tracker and CenpA - an inner kinetochore component. This cell line can be used to investigate microtubule engagement with kinetochores during metaphase.