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Advanced Mass Spectrometry

Our Capabilities

Application Areas:
  • Top-down Protein Analysis
  • Large Biomolecules Analysis
  • Protein and Peptide Sequencing
  • Post-Translational Modification Identification and Localisation
  • Conformer and Isomer Analysis
  • Lipidomics Analysis
  • DNA & RNA Analysis
  • Polymer Identification and Structural Analysis
  • Metabolomics Analysis and Structural Characterisation
  • Petroleomics Analysis
  • Environmental Studies
Routine Experiments that the Advance Mass Spectrometry RTP can offer are as follows:
  1. High resolution and high mass accuracy mass analysis - limited to ions from m/z 150 – 2500 - limited to masses up to 40 kDa - limited to the following solvent systems (H2O, MeCN, MeOH, EtOH, chloroform, dichloromethylene, IPA, toluene, hexane, acetone), but other solvents may work mostly depending on the purity of the solvent. - dual-spray internal calibration for guaranteed and traceable low-ppm to sub-ppm mass accuracy
    • Typical resolving power on FTICR-MS at 200 Da is > 1 million
    • Typical mass accuracy on FTICR-MS at 200 Da is < 0.1 ppm
    • Typical resolving power on FTICR-MS at 1000 Da is > 500,000
    • Typical mass accuracy on FTICR-MS at 1000 Da is < 0.3 ppm
  2. MS/MS of various molecular species
    • Collision-induced dissociation (CID)
    • Infrared multiphoton dissociation (IRMPD) (10 micron)
    • Ultraviolet photodissociation (UVPD) (213 nm, potentially 266 nm)
    • Electron capture dissociation (ECD) (Require multiply-charged positive ions)
    • Electron impact dissociation (EID)
    • Electron detachment dissociation (EDD) (negative ions)
    • Electron transfer dissociation (ETD)
    • Data dependent acquisition modes (DDA)
    • Data Independent Analysis (DIA)
    • Two-dimensional Mass Spectrometry (2DMS)
  3. Ion mobility mass spectrometry using trapped ion mobility spectrometry (TIMS) - can be combined with collision induced dissociation - possibly collision cross section measurement, with appropriate calibration
  4. Electrospray ionization (ESI) and nanoESI
  5. Matrix-assisted laser desorption/ionization (MALDI)
  6. Atmospheric pressure photoionization (APPI) and atmospheric pressure chemical ionization (APCI)
  7. Nanomate automated runs (96 samples at a time, but with a £500 extra consumables charge)
  8. Online LC-MS or GC-MS
  9. Offline fractionation using HPLC
Please contact us to discuss your samples.