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Events in MathSys and Complexity Science

This is a calendar page detailing events within the MathSys CDT. It also acts as a booking diary for the Seminar Room D1.07. To book D1.07 please email Sheetal.Sharma@warwick.ac.uk

Please note that your event booking is for D1.07 only. The adjacent common room is a private area for the MathSys Centre that cannot used as part of your booking.

MathSys CDT events have priority for D1.07 room bookings.

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Complexity Forum: Sascha Ott (Warwick)

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Location: D1.07 Complexity Science

Drop-seq at Warwick

Thanks to a donation we have recently purchased a device for single-cell transcriptome barcoding. Using it one can attach cell-specific molecular barcodes to mRNA sequences in about 5 percent of cells out of a sample of about 100k cells. These can then be processed and sequenced. The data coming out of this is essentially a matrix with read numbers for all genes in all successfully barcoded cells. These transcriptomes are typically very sparsely represented by few reads, but using techniques such as t-SNE (t-distributed stochastic neighbour embedding) related cells and cell types can readily be identified, cells can be grouped and data from many cells of the same type can be pooled. I am going to explain the device, present sample data sets, and discuss the statistical analysis.

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