Callan Littlejohn
PhD project
Evaluation of the capabilities of 2D Mass Spectrometry in a variety of molecular systems
External Partner
Verdel Instruments
Research
Working as part of the O'Connor Group, in the Millburn House ICR laboratory, my main focus is working on Two-Dimensional Mass Spectrometry, 2DMS is a truly data MS/MS independent technique that correlates fragment ions to their respective precursor m/z without the need for any isolation by passing ions through a fragmentation zone.
Current Research
Development of a universal method of 2DMS
A major issue with the adoption of 2DMS in a wider setting is the need for expensive equipment in order to perform the experiment. while versions using a Linear Ion Trap (LIT) are in development the vast majority of 2DMS is done in Fourier Transform Ion Cyclotron Resonance (FTICR) for which the price can run into the millions putting it out of reach for all but the best equipped labs.
In this work a new method of 2DMS was developed where the modulation occurs due to patterned fragmentation rather than radial excitation. The experiment was simulated using SIMION8.1 using a Linear TOF instrument as final analyser.
This meant that 2DMS could now be applied to every mass spectrometer as the technique is non-analyser specific. This was shown through further simulations where the technique was applied to a number of mass analysers.
This work was presented at ASMS 2022, IMSC 2022, BMSS 2022, and the PGR symposium at Warwick for which it won the prize for best oral in track. It was also the subject of a publication is JASMS in 2023.
Links
Publications
In-Silico Demonstration of Two Dimensional Mass Spectrometry using Spatially Dependent Fragmentation, JASMS, 2023 doi:https://doi.org/10.1021/jasms.2c00241
Conferences
BMSS Sept. 2021: gave a digital poster "Fellgett signal averaging in 2DMS"
ASCDT Sept 2021: gave a virtual presentation "Fellgett signal averaging in 2DMS"
TCT 3SIXTY sept 2021
Interplas sept 2021
LabInnovations Nov 2021
ASMS Jun 2022: poster "2DMS in a variety of systems"
ASCDT Jun 2022: poster "Short 2DMS of Covid-19"
EFTMS Jul 2022: Presentation "On the nature of Space charge: observations of dark charge effects"
IMSC Aug 2022: poster "2DMS in a variety of systems"
BMSS sept 2022: presentation: "2DMS in every mass analyser"
LabInnovations Nov 2022
Warwick Microbiology Cluster Meeting presentation "Two Dimensional Mass Spectrometry"
Warwick Chemistry Postgraduate symposium May 2023 presentation "Two Dimensional
Mass Spectrometry in every mass analyzer"
ASMS June 2023 Poster "Hyphenation of Trapped Ion Mobility with Two Dimensional Mass Spectrometry"
BMSS Sept 2023 Oral "Touchstone 2DMS: advanced Post-Processing for FTMS"
Grants and Prizes
John Beynon Travel grant: awarded by BMSS 2022
Nico Nibbering Student travel award awarded by IMSF 2022
Best Oral Presentation in stream B Warwick Postgraduate Symposium
John Beynon Travel grant awarded by BMSS 2023
Professional Memberships
Royal Society of Chemistry (AMRSC)
BMSS (Student Member)
ASMS (Student Member)
Get in touch!
Find me at Millburn House