I graduated in July 2012 from the University of Southampton with an MChem Masters degree in chemistry. During this time I completed an in-house project with Professor Malcolm Levitt studying the effects of paramagnetic materials on long lived singlet states in NMR. This year I have started studying for a PhD with Dr Marcel Utz, continuing at the University of Southampton.
NMR integrated microfluidic device for sensitive detection of cell metabolomics
The overall aim of my project is to develop a device that can be used to study the metabolites produced by cells at varying conditions. It should be able to be stored in a fluidic support hardware and be inserted wirelessly into the NMR spectrometer. A proposed structure of this device is shown on the right.
The device will be composed of three main layers:
- Fluidic Layer - This layer will contain the sample, ideally of cells.
- Pneumatic Layer - Due to the nature of the sample, this layer will provide the oxygen etc. that the cells require through a valve membrane.
- Resonator Layer - Detecting the signal of the nuclear spins. This electronic layer is optimised in order to provide maximum sensitivity, whilst maintaining sufficient resolution.
Long-term it is hoped that this device could be used to study the effect of changing conditions (e.g. addition of a drug molecule) to the metabolomics of cells.
Read All About It!
J. Bart, A. J. Kolkman, A. J. Oosthoek-de Vries, K. Koch, P. J. Nieuwland, H. J. W. G. Janssen, J. P. J. M. van Bentum, K. a M. Ampt, F. P. J. T. Rutjes, S. S. Wijmenga, H. J. G. E. Gardeniers, and A. P. M. Kentgens, Journal of the American Chemical Society, 2009, 131, 5014–5.