BolDFFS
Brassica oleracea Diversity Fixed Foundation Set (BolDFFS)
| The development of the BolDFFS was initiated with 376 founder accessions. |
| The collection is based on the EU GENRES core collection, with the addition of 25 fixed founder lines. |
| The 376 founder lines are divided into four subsets of 94 lines each, designated subsets 1-4, each subset corresponds to a microtitre plate with two control wells. |
 |
| Brassica oleracea diversity - grown in the field at Wellesbourne |
| Each subset has been selected to represent diversity within species subtaxa and crop type, as well as variation in eco-geographic origin. |
| The diversity set is being fixed through the generation of doubled haploid lines and inbreeding. The fixed lines can then replicated in field trials across labs and environments. |
| We have found that single seed descent of many B. oleracea lines, or selfing fixed lines results in very low seed yield due to self incompatibility. |
| As many lines did not respond to microspore culture, and fixing by SSD is not practical, a pragmatic decision was made to generate a smaller, but still representative set of fixed lines to represent the DFFS, i.e. up to 188 lines. |
For more information, see: Walley, P. G., Teakle, G. R., Moore, J. D., Allender, C. J., Pink, D. A. C, Buchanan-Wollaston, V., and Barker, G. (2012) Developing genetic resources for pre-breeding in Brassica oleracea: an overview of the UK perspective. Invited review, Journal of Plant Biotechnology, 30: 62-68  . |
| |
Seed |
| Accession information and seed requests should be made to Graham Teakle
|