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Genetic markers: RFLP


Work Flow:

  • Isolate DNA (a lot)
  • Restriction digest
  • Gel electrophoresis
  • Transfer fragments to nitrocellulose membrane filter
  • Use radio-labelled oligonucleotides (short DNA sequences) to probe the fragments
  • The labelled short DNA sequences that are complementary to the fragments allow visualisation on photographic paper (Southern blot).