Dr Alexander Darlington | Publications
All our papers our papers can be found online here.
Journal publications
[13] DP Byrom and APS Darlington# “Engineering feedback controllers to improve the evolutionary longevity of synthetic gene circuits” Nature Communications (accepted)
[12] J Kim*, APS Darlington*#, S Muñoz-Montero, R Montenegro, P Dalby, N Herrera-Martin, A Banks, S Prakash, K Polizzi, DG Bates and JI Jiménez# “Gene expression capacity is determined by the interplay between growth rate, resource biogenesis and nutrient quality.” ACS Synthetic Biology, 14(6), pp 2012-2029 doi: 10.1021/acssynbio.4c00828
[11] M Spurgeon, T Clark, TR Spartalis, APS Darlington, Xun Tang# and M Foo# “Designing biological network motif-based controllers by reverse engineering Hill function-type models from linear models” Royal Society Interface, 22, 20240811. doi: 10.1098/rsif.2024.0811
[10] A Mannan*, APS Darlington*#, RJ Tanaka and DG Bates# (2025) “Principles of engineering bacteria for efficient chemical production within limited cellular resources.” Nature Communications, 16, e279 doi: 10.1038/s41467-024-55347-y
>>> Warwick press release available online here and subject of an in depth article by The Chemical Engineer
[9] J Kim, S Lee, APS Darlington and J Kim (2024) “Resource reallocation in fleQ-deficient Pseudomonas putida enables consistent heterologous gene expression across various growth media.” Microbial Biotechnology, 17(11), e70054
[8] H Peng, APS Darlington, E South, HH Cohen, W Jiang and R Ledesma-Amaro (2024) “A characterized yeast toolkit for developing simple and complex microbial coculture systems.” Nature Microbiology, 9(3), pp. 848-863 doi: 10.1038/s41564-023-01596-4
>>> Imperial College London press release available online here.
[7] APS Darlington and DG Bates (2020) “Architectures for combined transcriptional and translational resource allocation controllers.” Cell Systems, 11(4), pp. 382-392. doi: 10.1016/j.cels.2020.08.014
[6] J Kim*, APS Darlington*, M Salvador, J Utrilla, and JI Jiménez (2020) “Trade-offs between gene expression, growth and phenotypic diversity in microbial populations.” Current Opinion in Biotechnology, 62, pp. 29-37. doi: 10.1016/j.copbio.2019.08.004
[5] APS Darlington, J Kim, and DG Bates (2019) “Robustness analysis of a synthetic translational resource allocation controller.” IEEE Control Systems Letters, 3(2), pp. 266-271. doi: 10.1109/LCSYS.2018.2867368
>>> Published in the special issue on Control and Network Theory for Biological Systems
[4] I Cazimoglu, APS Darlington, A Grigonyte, CEG Hoskin, J Liu, R, Oppenheimer, JA Siller-Farfan, R Carlise, C Grierson and A Papachristodoulou (2019) “Development of a Graduate Training Programme in Synthetic Biology: SynBioCDT.” OUP Synthetic Biology, 4(1), ysz006. doi: 10.1093/synbio/ysz006
>>> Published in a special issue on Education
[3] APS Darlington, J Kim, JI Jiménez, and DG Bates (2018) “Engineering translational resource allocation controllers: Mechanistic models, design rules and biological implementations.” ACS Synthetic Biology, 7(11), pp. 2485-2496. doi: 10.1021/acssynbio.8b00029
[2] APS Darlington*, J Kim*, JI Jiménez, and DG Bates (2018) “Dynamic allocation of orthogonal ribosomes facilitates uncoupling of co-expressed genes.” Nature Communications, 9, e695. doi: 10.1038/s41467-018-02898-6
>>> Warwick Press release available online here, picked up by over 20 new outlets worldwide.
[1] AW Cuthbert, M Murthy, and APS Darlington (2015) “Neural control of submucosal gland and apical membrane secretions in airways.” Physiological Reports 3(6) e12398. doi: 10.14814/phy2.12398
Peer-reviewed Conference proceedings
[10] DP Byrom and APS Darlington# “Selection of control inputs for enhancing the long-term performance of synthetic gene circuits.” Proc. of the 63nd IEEE Conference on Decision and Control, Milan, Italy. doi: 10.1109/CDC56724.2024.10886725
[9] APS Darlington#, A Mannan and D Bates “Comparison of closed loop control strategies for activating genetic switches.” Proc. of the 63nd IEEE Conference on Decision and Control, Milan, Italy. doi: 10.1109/CDC56724.2024.10886005.
[8] DP Byrom and APS Darlington#. “On the implications of controller resource consumption for the long-term performance of synthetic gene circuits.” Proc. of the 62nd IEEE Conference on Decision and Control, Singapore. doi: 10.1109/CDC49753.2023.10383537.
[7] APS Darlington#, A Mannan, and DG Bates. “Natural host feedback simplifies the design of metabolic switches.” Proc. of the 62nd IEEE Conference on Decision and Control, Singapore. doi: 10.1109/CDC49753.2023.10383416.
[6] APS Darlington and DG Bates (2022) “Performance and robustness analysis of control strategies for ameliorating cellular host-circuit interactions” Proc. of the 20th IEEE European Control Conference, London, UK. doi: 10.23919/ECC55457.2022.9837985
[5] APS Darlington and DG Bates (2020) “Robustness analysis of combined transcriptional and translational resource allocation controllers.” Proc. of the 21st IFAC World Congress, Berlin, Germany
[4] APS Darlington and DG Bates (2019) “Combining transcriptional and translational resource allocation controllers for synthetic circuits.” Proc. of the 58th IEEE Conference on Decision and Control, Nice, France. doi: 10.1109/CDC40024.2019.9028910
[3] APS Darlington and DG Bates (2018) “On the design of integral translational resource allocation controllers for synthetic cellular circuitry.” Proc. of the 57th IEEE Conference on Decision and Control, Miami, FL, USA. doi: 10.1109/CDC.2018.8618723
[2] APS Darlington, J Kim, JI Jiménez and DG Bates (2017) “Design of a translational resource allocation controller to manage cellular resource limitations.” Proc. of the 20th World Congress of the International Federation of Automatic Control, Toulouse, France available as IFAC-PapersOnLine, 50 (1), pp. 12653-12660. doi: 10.1016/j.ifacol.2017.08.2239
[1] APS Darlington and DG Bates (2016) “Host-aware modelling of a synthetic genetic oscillator.” Proc. of the 38th IEEE Engineering in Medicine and Biology Conference, Orlando, FL, USA. doi: 10.1109/EMBC.2016.7590985