Abstract: My lab is interested in how nucleosomes are assembled from their precursors and the implications this has on genomic processes. Specifically, we are interested in how proteins known as histone chaperones function to assemble chromatin, and, in addition to more traditional biochemical approaches, we have been developing ways to observe this in living cells. Alongside this, serendipity has led us into the field of high-order chromatin structure, where we are using replication-coupled pulse-labelling of fluorescent histones to observe the dynamics of chromatin architecture using lattice light-sheet microscopy. Because these projects are equally exciting, time permitting, I shall talk a little bit about both.