My research interests lie in the understanding the molecular and physical principles that govern processes at the plasma membrane of cells. Particularly, by which mechanisms the force generating machinery of the cell cortex, structural filaments and motor proteins, govern and regulate the mechanical properties of the cell membrane and dynamics of cell membrane components, and vice versa, how membrane organisation and signalling events feed-back to the regulation of the cortex machinery. These mechanisms, which in turn regulate cell motility and cell-cell interactions, underlie important, poorly-understood human diseases that constitute global health problems.
To study these processes systematically, our lab employs a combination of in vitro, reconstituted minimal systems and experiments on cultured cells to dissect and understand the interlinked contributions of membrane mechanics, cytoskeletal activity and membrane organisation. We use quantitative imaging approaches, controlled mechanical and biochemical manipulations and work with theoretical physicists to unravel the unsolved puzzle of mechano-sensing and cell membrane tension regulation.
1. Mosby, L.S., et al., Visualizing acto-myosin dynamics at a membrane surface using interferometric scattering microscopy, Biophys J., 2017, doi: 10.1016/j.bpj.2020.02.025
2. Das, A., et al., Stratification relieves constraints from steric hindrance in the generation of compact actomyosin asters at the membrane cortex, Science Advances, 2020, doi: 10.1126/sciadv.aay6093
3. Ditlev, J.A., Vega, A.R. #, Köster, D.V. et al, A Composition-Dependent Molecular Clutch Between T Cell Signaling Clusters and Actin. eLife 2019, doi: 10.7554/eLife.42695
4. Köster DV, et al., Actomyosin dynamics drive local membrane component organization in an in vitro active composite layer. Proc. Natl. Acad. Sci. 2016, doi:10.1073/pnas.1514030113.
5. Sinha B, Köster D, et al., Cells respond to mechanical stress by rapid disassembly of caveolae. Cell 2011, doi: 10.1016/j.cell.2010.12.031