We are interested in two processes fundamental to eukaryotic cellular life: mitosis and membrane trafficking. The lab are working to understand how these processes operate at the molecular level in the hope of identifying new strategies to prevent tumour formation and propagation in cancer and to inhibit cellular infection.
The mitotic spindle is a tiny machine whose purpose is to accurately segregate the chromosomes during cell division. We are trying to understand how the microtubules of the mitotic spindle are stabilised by non-motor proteins, and how stability is altered in cancer.
Clathrin-mediated endocytosis is the main route of entry into mammalian cells and it controls numerous cellular processes from cell motility and signalling to synaptic transmission and organelle identity. We are trying to understand the molecular mechanisms of this process, and how it is controlled by the cell cycle.
Nixon, F.M., Gutiérrez-Caballero, C., Hood, F.E., Booth, D.G., Prior, I.A. & Royle, S.J. (2015) The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle eLife, 4: e07635. doi: 10.7554/eLife.07635
Kaur, S., Fielding, A.B., Gassner, G., Carter, N.J. & Royle, S.J. (2014) An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis eLife 3: e00829. doi: 10.7554/eLife.00829
Hood, F.E., Williams, S.J., Burgess, S.G., Richards, M.W., Roth, D., Straube, A., Pfuhl, M., Bayliss**, R. & Royle**, S.J. (2013) Coordination of adjacent domains mediates TACC3–ch-TOG–clathrin assembly and mitotic spindle binding. J. Cell Biol. 202: 463-78. doi: 10.1083/jcb.201211127
Cheeseman, L.P., Harry, E.F., McAinsh, A.D., Prior, I.A., Royle, S.J. (2013) Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. J. Cell Sci. 126: 2102-13. doi: 10.1242/jcs.124834