Vitmain C content at harvest (Day 0) was quantified using a analytical HPLC approach. The first aim of this was to evaluate the natural variation in vitamin C within the mapping population (a), secondly to estabilish if vitamin C levels correlated with yellowing phenotypes (b) and thirdly to map vitamin C QTLs if lines varied significantly (see Results: QTL analysis).
As you can see from the bar chart, the mapping population displays a high level of natural variation for vitamin C content at harvest. All lines meet or exceed the recommended daily allowance (60mg) for vitamin C in a 100g portion. Vitamin C content does not correlate with time taken to yellow (Days), suggesting that each of these phenotypic traits are under separate genetic control.