3i SDC methods reporting
| Microscope (Make, Model) | 3i, Marianas SDC |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Zeiss, 100x, 1.46, oil, alphaPlnApo Zeiss, 63x, 1.40, oil, Pln Apo Zeiss, 40x, 1.30, oil, EC PlnN Zeiss, 20x, 0.5, air, EC PlnN |
| Light source(s) |
Select the one/s used: 405 nm, 488 nm, 561 nm, 647 nm lasers |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Dichroic: 405/488/561/640 nm Emission filters (Mode 4): c405s - 445/45 c488s - 525/30 c561s - 617/73 c640s - 692/40 Quad - 446/523/600/677 Information on other filters are available on request. |
| fpbase.org channel set-up | https://www.fpbase.org/microscope/aomAJq4qBQJFYRhWcVvdQ9/ |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Yokogawa CSU X-10 spinning disk unit Zeiss Definite Focus 2 autofocus Okolab microscope incubator ASI motorised xy stage ASI 300 um z-piezo 3i Ablate! laser ablation system (532 nm, 1.3 ns pulsed laser) 3i Vector photomanipulation unit |
| Acquisition software and settings | SlideBook v6.0 |
| Camera manufacturer, model | 2 x Photometrics 95B Prime |