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3i SDC methods reporting

Microscope (Make, Model) 3i, Marianas SDC
Inverted or upright Inverted
Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any)

Select the one/s used:

Zeiss, 100x, 1.46, oil, alphaPlnApo

Zeiss, 63x, 1.40, oil, Pln Apo

Zeiss, 40x, 1.30, oil, EC PlnN

Zeiss, 20x, 0.5, air, EC PlnN

Light source(s)

Select the one/s used:

405 nm, 488 nm, 561 nm, 647 nm lasers

Filters, dichroic mirrors, beam splitters

Select the one/s used:

Dichroic: 405/488/561/640 nm

Emission filters (Mode 4):

c405s - 445/45

c488s - 525/30

c561s - 617/73

c640s - 692/40

Quad - 446/523/600/677

Information on other filters are available on request. channel set-up
Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective

Yokogawa CSU X-10 spinning disk unit

Zeiss Definite Focus 2 autofocus

Okolab microscope incubator

ASI motorised xy stage

ASI 300 um z-piezo

3i Ablate! laser ablation system (532 nm, 1.3 ns pulsed laser)

3i Vector photomanipulation unit

Acquisition software and settings SlideBook v6.0
Camera manufacturer, model 2 x Photometrics 95B Prime